Sino Biological™ MCAM Recombinant Human Protein, His Tag

This recombinant protein was expressed from a DNA sequence encoding the extracellular domain of human MCAM precursor (NP_006491.2) (Met 1-Gly 559) fused to a C-terminal polyhistidine tag

Overview
Brand: Invitrogen

Manufacturer Part Number: 10115H08H25

RECOM HU MCAM (HIS)

UNSPSC: 12352200

Code: NEW

Additional Details:
Additional Details: Weight: 0.10000kg



Disclaimers: For Research Use Only. Not for use in diagnostic procedures.

Product Code. 15204675

Quantity Price
1 £ 383.0 / 25µg
Estimated Shipment date
from Supplier 23-12-2016
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Description and Specification

Specification

For Use With (Application) Proteins, Expression, Isolation & Analysis; Enzyme & Protein Activity Assays; Protein Assay Controls, Reference Standards & Accessories
Format Lyophilized
Molecular Weight 61.3kDa
Purity ≥90% by SDS-PAGE
Quantity 5 x 5μg
Storage Requirements Store at -20°C. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles.Expires one year from date of receipt when stored as instructed.
Protein Subtype Intercellular Adhesion Molecule
Endotoxin Concentration <1 EU/μg
Gene Alias MCAM, CD146, MUC18
Common Name Melanoma Cell Adhesion Molecule
Gene Symbol MCAM
Protein Tag His Tag
Validated Application Assay Standard
Protein Family CD Proteins
Expression System Mammalian

Melanoma Cell Adhesion Molecule (MCAM), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in analysis of protein structure, and performing cell based assays. It can also be used as an immunogen, as a protein standard, or in other research applications.
  • Activity: Measured by the ability of the immobilized protein to support the adhesion of the HUVEC human umbilical vein endothelial cell line; when 3 x 104 cells/well are added to human MCAM coated plates (0.8μg/mL, 100μL/well), approximately >50% will adhere after one hour at 37°C
  • Formulation: Lyophilized in 140mM NaCl, 2.7mM KCl, 10mM Na2HPO4, 1.8mM KH2PO4, pH 7.4, 5% Mannitol, 5% Trehalose, 0.02% Tween 80
  • Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2mg/mL or lower; aliquot and use as soon as possible

This protein is manufactured by Sino Biological Inc.