Sino Biological™ MCAM (CD146) Recombinant Human Protein, hIgG1-Fc Tag

This recombinant protein was expressed from a DNA sequence encoding the extracellular domain (Met 1-Gly 559) of human MCAM precursor (NP_006491.2) fused to the Fc region of human IgG1 at the C-terminus

Overview
Brand: Invitrogen

Manufacturer Part Number: 10115H02H50

UNSPSC: 12352202

Code: NEW

Additional Details:
Additional Details: Weight: 0.01000kg



Disclaimers: For Research Use Only. Not for use in diagnostic procedures.

Product Code. 13415726

Quantity Price
1 £ 357.0 / 50µg
Estimated Shipment date
from Supplier 13-03-2017
Add to basket


Description and Specification

Specification

Storage Requirements Store at -20° to -70°C. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles. Expires one year from date of receipt when stored as instructed.
Quantity 50μg
For Use With (Application) Proteins, Expression, Isolation & Analysis; Enzyme & Protein Activity Assays; Protein Assay Controls, Reference Standards & Accessories
Format Lyophilized
Expression System Mammalian
Molecular Weight 86.4kDa
Purity ≥90% by SDS-PAGE
Validated Application Assay Standard
Protein Family CD Proteins
Endotoxin Concentration <1 EU/μg
Protein Subtype Other Proteins
Structural Form Truncated/Recombinant
Gene Alias MCAM, CD146, MUC18
Common Name Melanoma cell adhesion molecule
Protein Tag IgG1 Fc Tag
Gene Symbol MCAM
Gene ID (Entrez) 4162

Melanoma cell adhesion molecule (MCAM/CD146), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in cell biology research applications. It can also be used as a protein standard.
  • Activity: Measured by the ability of the immobilized protein to support the adhesion of the HUVEC human umbilical vein endothelial cell line; when 3 x 104 cells/well are added to human CD146 coated plates (0.8μg/mL, 100μL/well), approximately >30 % will adhere after one hour at 37°C
  • Formulation: Lyophilized in PBS, pH 7.4, 5% Mannitol, 5% Trehalose, and 0.02% Tween 80
  • Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2mg/mL or lower; aliquot and use as soon as possible

Shipping Condition: Room Temperature. This protein is manufactured by Sino Biological Inc.