Sino Biological™ LEPR Recombinant Human Protein, His Tag

This recombinant protein was expressed from a DNA sequence encoding the extracellular domain (Met 1-Asp 839) of human LEPR (NP_002294.2) fused to a polyhistidine tag at the C-terminus

Brand:  Invitrogen 10322H08H5

Product Code. 15255155

Disclaimers:

For Research Use Only. Not for use in diagnostic procedures.

Description

Leptin receptor (LEPR), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in protein studies such as protein structure analysis and protein-protein interactions. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications.

• Activity: Measured by its binding ability in a functional ELISA; immobilized human LEPR at 5μg/mL (100μL/well) can bind human leptin receptor (LEPR) with a linear range of 0.032-4.0μg/mL
• Formulation: Lyophilized in 140mM NaCl, 2.7mM KCl, 10mM Na₂HPO₄, 1.8mM KH₂PO₄, pH 7.4, 5% Mannitol, 5% Trehalose, 0.02% Tween 80
• Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2mg/mL or lower; aliquot and use as soon as possible

This protein is manufactured by Sino Biological Inc.

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Specifications

• For Use With (Application): Proteins, Expression, Isolation & Analysis; Enzyme & Protein Activity Assays; Protein Assay Controls, Reference Standards & Accessories
• Molecular Weight: 95kDa
• Quantity: 5μg
• Protein Subtype: Cytokine Receptors|Hormone Receptors
• Gene Alias: LEPR, CD295, OBR
• Gene Symbol: LEPR
• Protein Family: Other Proteins
• Protein Tag: His Tag

• Format: Lyophilized
• Purity: ≥95% by SDS-PAGE
• Storage Requirements: Store at -20°C. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles. Expires one year from date of receipt when stored as instructed.
• Endotoxin Concentration: <1 EU/μg
• Common Name: Leptin receptor
• Validated Application: Assay Standard
• Expression System: Mammalian