Sino Biological™ LEP (Leptin) Recombinant Human Protein

This recombinant protein was expressed from a DNA sequence encoding the mature form of human Leptin (NP_000221.1), residues 22 to 167, containing an additional Met

Overview
Brand: Invitrogen

Manufacturer Part Number: 10221HNAE50

UNSPSC: 12352202

Code: NEW

Additional Details:
Additional Details: Weight: 0.01000kg



Disclaimers: For Research Use Only. Not for use in diagnostic procedures.

Product Code. 13452949

Quantity Price
1 £ 62.0 / 50µg
Estimated Shipment date
from Supplier 10-04-2017
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Description and Specification

Specification

Gene Symbol LEP
Protein Subtype Lectins & Leptins
Common Name Leptin
Molecular Weight 16kDa
Purity ≥95% by SDS-PAGE
For Use With (Application) Proteins, Expression, Isolation & Analysis; Enzyme & Protein Activity Assays; Protein Assay Controls, Reference Standards & Accessories
Quantity 50μg
Storage Requirements Store at -20° to -70°C. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles. Expires one year from date of receipt when stored as instructed.
Format Lyophilized
Protein Tag Untagged
Protein Family Other Proteins
Gene ID (Entrez) 3952
Structural Form Native
Gene Alias FLJ94114, OB, OBS
Validated Application Assay Standard
Expression System E. coli

Leptin (LEP), recombinant human protein is supplied as a lyophilized powder. In general, recombinant proteins are suitable for use in studying protein-protein interactions, as a protein standard, or in cell biology research applications.
  • Activity: Measured by its binding ability in a functional ELISA: 1. Immobilized human Leptin at 1.25μg/mL (100μL/well) can bind human Leptin receptor-Fc with a linear range of 0.032-4.0μg/mL; 2. Immobilized human Leptin at 5μg/mL (100μL/well) can bind human Leptin receptor-His with a linear range of 0.032-4.0μg/mL
  • Formulation: Lyophilized in 140mM NaCl, 2.7mM KCl, 10mM Na2HPO4, 1.8mM KH2PO4, pH 7.4, 5% Mannitol, 5% Trehalose, 0.02% Tween 80
  • Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2mg/mL or lower; aliquot and use as soon as possible

This protein is manufactured by Sino Biological Inc.