Sino Biological™ KITL Recombinant Mouse Protein, His Tag

This recombinant protein was expressed from a DNA sequence encoding the mouse KITL (P20826-1) (Met 1-Ala 189) fused to a polyhistidine tag at the C-terminus

Overview
Brand: Invitrogen

Manufacturer Part Number: 50487M08B50

UNSPSC: 12352202

Code: NEW

Additional Details:
Additional Details: Weight: 0.01000kg



Disclaimers: For Research Use Only. Not for use in diagnostic procedures.
Includes: One vial of lyophilized protein

Product Code. 13443149

Quantity Price
1 £ 353.0 / 50µg
Estimated Shipment date
from Supplier 07-04-2017
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Description and Specification

Specification

Quantity 50μg
Storage Requirements Store at -20°C. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles. Expires one year from date of receipt when stored as instructed.
Format Lyophilized
Validated Application Assay Standard
Protein Subtype Other Growth Factors
For Use With (Application) Proteins, Expression, Isolation & Analysis; Enzyme & Protein Activity Assays; Protein Assay Controls, Reference Standards & Accessories
Structural Form Truncated/Recombinant/Cell Surface Protein
Endotoxin Concentration <1 EU/μg
Gene Symbol KITL
Expression System Insect
Protein Family Growth Factors & Receptors
Molecular Weight 19.7kDa
Common Name Kit ligand
Purity ≥90% by SDS-PAGE
Protein Tag His Tag
Gene Alias Clo, Con, Gb, Kitlg, mgf, SCF, SF, SLF, Sl, Steel, c ontrasted

KIT ligand (KITL), recombinant mouse protein is supplied as a lyophilized powder. It is suitable for use in analysis of protein structure, and performing cell based assays. It can also be used as an immunogen, as a protein standard, or in other research applications.
  • Activity: 1. Measured by its binding ability in a functional ELISA; immobilized mouse KITL at 2μg/mL (100μL/well) can bind mouse KIT with a linear ranger of 1.28-32ng/mL; 2. Measured in a cell proliferation assay using TF-1 human erythroleukemic cells (Kitamura, T. et al. 1989, J. Cell Physiol. 140:323), the EC50 for this effect is typically 0.5-5ng/mL
  • Formulation: Lyophilized in 20mM Tris, 500mM NaCl, pH 7.4, 5% Mannitol, 5% Trehalose, 0.02% Tween 80
  • Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2mg/mL or lower; aliquot and use as soon as possible

This protein is manufactured by Sino Biological Inc.