Sino Biological™ INSR Recombinant Human Protein (Long Isoform, without Catalytic Activity Domain), His Tag

This recombinant protein was expressed from a DNA sequence encoding the extracellular domain (Met 1-Lys 956) of human INSR long isoform (NP_000199.2) fused to a polyhistidine tag at the C-terminus

Brand:  Invitrogen 11081H08H50

Product Code. 13496086

Disclaimers:

For Research Use Only. Not for use in diagnostic procedures.

Description

Insulin receptor (INSR), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in protein studies such as protein structure analysis and protein-protein interactions. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications. Since this product is a truncated protein and does not contain intracellular catalytic activity domain, it is not suitable for use in enzyme activity studies.

• Activity: Measured by its binding ability in a functional ELISA; when 1μg/mL of biotinylated human insulin is immobilized onto a streptavidin coated plate, it can bind human INSR, isoform long, with a linear range of 0.625-10μg/mL
• Formulation: Lyophilized in PBS, pH 7.4, 5% Mannitol, 5% Trehalose, and 0.02% Tween-80
• Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2mg/mL or lower; aliquot and use as soon as possible

Shipping Condition: Room Temperature. This protein is manufactured by Sino Biological Inc.

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Specifications

• For Use With (Application): Proteins, Expression, Isolation & Analysis; Enzyme & Protein Activity Assays; Protein Assay Controls, Reference Standards & Accessories
• Gene ID (Entrez): 3643
• Purity: ≥85% by SDS-PAGE
• Storage Requirements: Store at -20° to -70°C. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles. Expires one year from date of receipt when stored as instructed.
• Protein Subtype: Hormone Receptors
• Gene Alias: CD220, HHF5, INSR
• Gene Symbol: INSR
• Protein Family: Growth Factors & Receptors
• KinaseFamily: Insulin & IGF Receptor Kinase Family

• Format: Lyophilized
• Molecular Weight: 107.0kDa
• Quantity: 50μg
• Structural Form: Truncated/Recombinant/Cell Surface Protein
• Endotoxin Concentration: <1 EU/μg
• Common Name: Insulin receptor, long isoform
• Validated Application: Assay Standard
• Expression System: Mammalian
• Protein Tag: His Tag