Sino Biological™ IGFBP3 Recombinant Human Protein, His Tag

This recombinant protein was expressed from a DNA sequence encoding the mature form of human IGFBP3 (NP_000589.2), residues 28 to 291, fused to a polyhistidine tag at the N-terminus

Overview
Brand: Invitrogen

Manufacturer Part Number: 10430H07H5

IGFBP3 Recombinant Human Protein (His Tag, Active)5 µg

UNSPSC: 12352202

Code: NEW

Additional Details:
Additional Details: Weight: 0.01000kg



Disclaimers: For Research Use Only. Not for use in diagnostic procedures.

Product Code. 13465876

Quantity Price
1 £ 66.0 / 5µg
Estimated Shipment date
from Supplier 13-12-2016
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Description and Specification

Specification

For Use With (Application) Proteins, Expression, Isolation & Analysis; Enzyme & Protein Activity Assays; Protein Assay Controls, Reference Standards & Accessories
Format Lyophilized
Gene ID (Entrez) 3486
Molecular Weight 31kDa
Purity ≥85% by SDS-PAGE
Quantity 5μg
Storage Requirements Store at -20° to -70°C. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles. Expires one year from date of receipt when stored as instructed.
Structural Form Recombinant
Protein Subtype IGFs (Insulin-Like Growth Factors)
Endotoxin Concentration <1 EU/μg
Gene Alias IGFBP3, tcag7.703, BP-53, IBP3
Common Name Insulin-like growth factor binding protein 3
Gene Symbol IGFBP3
Protein Tag His Tag
Validated Application Assay Standard
Protein Family Growth Factors & Receptors
Expression System Mammalian

Insulin-like growth factor binding protein 3 (IGFBP3), recombinant human protein is supplied as a lyophilized powder. In general, recombinant proteins are suitable for use in studying protein-protein interactions, as a protein standard, or in cell biology research applications.
  • Activity: Measured by its binding ability in a functional ELISA: 1. Immobilized human IGF2 at 10μg/mL (100μL/well) can biotinylated human IGFBP3-His, the ED50 of can biotinylated human IGFBP3-His is 18ng/mL; 2. Immobilized human IGF1 at 10μg/mL (100μL/well) can biotinylated human IGFBP3-His, the ED50 of can biotinylated human IGFBP3-His is 24ng/mL
  • Formulation: Lyophilized in PBS, pH 7.4, 5% Mannitol, 5% Trehalose, and 0.02% Tween 80
  • Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2mg/mL or lower; aliquot and use as soon as possible

Shipping Condition: Room Temperature. This protein is manufactured by Sino Biological Inc.