Sino Biological™ HTRA2 Recombinant Human Protein, His Tag

This recombinant protein was expressed from a DNA sequence encoding the mature form of human HTRA2 (O43464-1) (Ala 134-Glu 458) fused to a polyhistide tag at the C-terminus

Overview
Brand: Invitrogen

Manufacturer Part Number: 10619H08E25

HTRA2 RECOMBINANT HUMAN PROTEIN, HIS TAG

UNSPSC: 12352202

Code: NEW

Additional Details:
Additional Details: Weight: 0.10000kg



Disclaimers: For Research Use Only. Not for use in diagnostic procedures.

Product Code. 15216995

Quantity Price
1 £ 122.0 / 25µg
Estimated Shipment date
from Supplier 09-12-2016
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Description and Specification

Specification

For Use With (Application) Proteins, Expression, Isolation & Analysis; Enzyme & Protein Activity Assays; Protein Assay Controls, Reference Standards & Accessories
Format Frozen
Molecular Weight 36.5kDa
Purity ≥85% by SDS-PAGE
Quantity 5 x 5μg
Storage Requirements Store at -80°C. Avoid repeated freeze-thaw cycles. Expires one year from date of receipt when stored as instructed.
Protein Subtype Proteases
Gene Alias OMI, PARK13, PRSS25
Common Name HtrA serine peptidase 2
Gene Symbol HTRA2
Protein Tag His Tag
Validated Application Assay Standard
Expression System E. coli

HTRA Serine Peptidase 2 (HTRA2), recombinant human protein is supplied in frozen format. It is suitable for use in protein studies such as enzymatic analysis and protein-protein interactions. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications.
  • Activity: Protease activity demonstrated by HTRA2 cleavage of bovine beta-casein (Sigma Cat. No. C-6905); incubation of beta-casein at 0.2μg/mL with Recombinant Human HTRA2 at 0.02μg/mL (ratio of 10:1) for 60 minutes at 45°C in 50mM Tris, pH 8.0, which results in >95% cleavage of beta-casein, as revealed by SDS-PAGE
  • Formulation: 50mM Tris, 0.3M NaCl, 1mM DTT, 0.2μg/μL BSA, 20% glycerol, pH 7.8
  • Reconstitution: No reconstitution needed

This protein is manufactured by Sino Biological Inc.