FLT1 (VEGFR1) Recombinant Human Protein (without Catalytic Activity Domain), His Tag, Invitrogen™ Sino Biological™

This recombinant protein was expressed from a DNA sequence encoding the extracellular domain (Met 1-Asn 756) of human FLT1 (P17948-1) fused to a polyhistidine tag at the C-terminus

Brand:  Invitrogen 10136H08H5

Product Code. 13485726

Description

Fms-related tyrosine kinase 1 (FLT1/VEGFR1), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in analysis of protein structure. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications. Since this product is a truncated protein and does not contain intracellular catalytic activity domain, it is not suitable for use in enzyme activity studies.

• Activity: Measured by its ability to inhibit the VEGF-dependent proliferation of human umbilical vein endothelial cells (HUVEC) (Conn, G. et al., 1990 , Proc. Natl. Acad. Sci. USA 87:1323); the ED₅₀ for this effect is typically 5-30ng/mL in the presence of 5ng/mL human VEGF165
• Formulation: Lyophilized in PBS, pH 7.4, 5% Mannitol, 5% Trehalose, and 0.02% Tween-80
• Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2mg/mL or lower; aliquot and use as soon as possible

Shipping Condition: Room Temperature. This protein is manufactured by Sino Biological Inc.

Specifications

• For Use With (Application): Protein Expression and Isolation, Enzyme and Protein Activity Assay, Protein Assay Control, Reference Standards and Accessories
• Molecular Weight (g/mol): 83.7kDa
• Storage Requirements: Store at -20° to -70°C. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles. Expires one year from date of receipt when stored as instructed.
• Endotoxin Concentration: <1 EU/μg
• Common Name: fms-related tyrosine kinase 1
• KinaseFamily: VEGF Receptors
• Expression System: Mammalian
• Form: Lyophilized
• Purity or Quality Grade: ≥85% by SDS-PAGE

• Gene ID (Entrez): 2321
• Quantity: 5μg
• Structural Form: Truncated/Recombinant
• Gene Alias: FLT, VEGFR1, FLT1
• Gene Symbol: FLT1
• Protein Tag: His Tag
• Protein Family: Growth Factors & Receptors
• Protein Subtype: Receptor Tyrosine Kinases, Growth Factor Receptors

For Research Use Only. Not for use in diagnostic procedures.