Sino Biological™ FLT1 (VEGFR1) Recombinant Human Protein (without Catalytic Activity Domain), His Tag

This recombinant protein was expressed from a DNA sequence encoding the extracellular domain (Met 1-Asn 756) of human FLT1 (P17948-1) fused to a polyhistidine tag at the C-terminus

Overview
Brand: Invitrogen

Manufacturer Part Number: 10136H08H5

FLT1 (VEGFR1) Recombinant Human Protein (His Tag,Active), 5 µg

UNSPSC: 12352202

Code: NEW

Additional Details:
Additional Details: Weight: 0.01000kg



Disclaimers: For Research Use Only. Not for use in diagnostic procedures.

Product Code. 13485726

Quantity Price
1 £ 54.5 / 5µg
Estimated Shipment date
from Supplier 07-12-2016
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Description and Specification

Specification

For Use With (Application) Proteins, Expression, Isolation & Analysis; Enzyme & Protein Activity Assays; Protein Assay Controls, Reference Standards & Accessories
Format Lyophilized
Gene ID (Entrez) 2321
Molecular Weight 83.7kDa
Purity ≥85% by SDS-PAGE
Quantity 5μg
Storage Requirements Store at -20° to -70°C. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles. Expires one year from date of receipt when stored as instructed.
Structural Form Truncated/Recombinant
Protein Subtype Receptor Tyrosine Kinases|Growth Factor Receptors
Endotoxin Concentration <1 EU/μg
Gene Alias FLT, VEGFR1, FLT1
Common Name fms-related tyrosine kinase 1
Gene Symbol FLT1
Protein Tag His Tag
Validated Application Assay Standard
Protein Family Growth Factors & Receptors
Expression System Mammalian
KinaseFamily VEGF Receptors

Fms-related tyrosine kinase 1 (FLT1/VEGFR1), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in analysis of protein structure. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications. Since this product is a truncated protein and does not contain intracellular catalytic activity domain, it is not suitable for use in enzyme activity studies.
  • Activity: Measured by its ability to inhibit the VEGF-dependent proliferation of human umbilical vein endothelial cells (HUVEC) (Conn, G. et al., 1990 , Proc. Natl. Acad. Sci. USA 87:1323); the ED50 for this effect is typically 5-30ng/mL in the presence of 5ng/mL human VEGF165
  • Formulation: Lyophilized in PBS, pH 7.4, 5% Mannitol, 5% Trehalose, and 0.02% Tween-80
  • Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2mg/mL or lower; aliquot and use as soon as possible

Shipping Condition: Room Temperature. This protein is manufactured by Sino Biological Inc.