Thermo Scientific™ Sino Biological™ FLT1 (VEGFR1) Recombinant Human Protein (without Catalytic Activity Domain), His Tag
This recombinant protein was expressed from a DNA sequence encoding the extracellular domain (Met 1-Asn 756) of human FLT1 (P17948-1) fused to a polyhistidine tag at the C-terminus
Brand: Thermo Scientific™ 10136H08H250
Code : NEW
Additional Details : Weight : 0.01000kg
DescriptionFms-related tyrosine kinase 1 (FLT1/VEGFR1), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in analysis of protein structure. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications. Since this product is a truncated protein and does not contain intracellular catalytic activity domain, it is not suitable for use in enzyme activity studies.
- Activity: Measured by its ability to inhibit the VEGF-dependent proliferation of human umbilical vein endothelial cells (HUVEC) (Conn, G. et al., 1990 , Proc. Natl. Acad. Sci. USA 87:1323); the ED50 for this effect is typically 5-30ng/mL in the presence of 5ng/mL human VEGF165
- Formulation: Lyophilized in PBS, pH 7.4, 5% Mannitol, 5% Trehalose, and 0.02% Tween-80
- Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2mg/mL or lower; aliquot and use as soon as possible
Shipping Condition: Room Temperature. This protein is manufactured by Sino Biological Inc.
|Proteins, Expression, Isolation & Analysis; Enzyme & Protein Activity Assays; Protein Assay Controls, Reference Standards & Accessories|
|≥85% by SDS-PAGE|
|Store at -20° to -70°C. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles. Expires one year from date of receipt when stored as instructed.|
|Receptor Tyrosine Kinases|Growth Factor Receptors|
|FLT, VEGFR1, FLT1|
|Growth Factors & Receptors|
|5 x 50μg|
|fms-related tyrosine kinase 1|
For Research Use Only. Not for use in diagnostic procedures.