EGF Recombinant Mouse Protein, hIgG1-Fc Tag, Invitrogen™ Sino Biological™

This recombinant protein was expressed from a DNA sequence encoding the mature form of mouse EGF (P01132) (Asn 977-Arg 1029) fused to the Fc region of human IgG1 at the N-terminus

Includes: Five vials of lyophilized protein

Product Code	Brand	Quantity
13493139	Invitrogen 50482M01H5	5μg
13473139	Invitrogen 50482M01H25	5 x 5μg

Description

Epidermal growth factor (EGF), recombinant mouse protein is supplied as a lyophilized powder. It is suitable for use in performing cell based assays. It can also be used as a protein standard, or in other research applications.

• Activity: Measured in a cell proliferation assay using BALB/c 3T3 mouse embryonic fibroblasts; the EC₅₀ for this effect is typically 0.2-1ng/mL
• Formulation: Lyophilized in 140mM NaCl, 2.7mM KCl, 10mM Na₂HPO₄, 1.8mM KH₂PO₄, pH 7.4, 5% Mannitol, 5% Trehalose, 0.02% Tween 80
• Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2mg/mL or lower; aliquot and use as soon as possible

This protein is manufactured by Sino Biological Inc.

Specifications

• For Use With (Application): Protein Expression and Isolation, Enzyme and Protein Activity Assay, Protein Assay Control, Reference Standards and Accessories
• Storage Requirements: Store at -20°C. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles. Expires one year from date of receipt when stored as instructed.
• Endotoxin Concentration: <1 EU/μg
• Common Name: Epidermal growth factor
• Protein Tag: IgG1 Fc Tag
• Protein Family: Growth Factors & Receptors
• Protein Subtype: EGF (Epidermal Growth Factor)

• Molecular Weight (g/mol): 34.5kDa
• Structural Form: Full Length/Recombinant
• Gene Alias: AI790464
• Gene Symbol: EGF
• Expression System: Mammalian
• Form: Lyophilized
• Purity or Quality Grade: ≥90% by SDS-PAGE

For Research Use Only. Not for use in diagnostic procedures.