Sino Biological™ EGF Recombinant Mouse Protein, hIgG1-Fc Tag

This recombinant protein was expressed from a DNA sequence encoding the mature form of mouse EGF (P01132) (Asn 977-Arg 1029) fused to the Fc region of human IgG1 at the N-terminus

Overview
Brand: Invitrogen

Manufacturer Part Number: 50482M01H250

EGF Rec. Ms. Protein (hIgG1-Fc Tag, Act.), 250 µg

UNSPSC: 12352202

Code: NEW

Additional Details:
Additional Details: Weight: 0.01000kg



Disclaimers: For Research Use Only. Not for use in diagnostic procedures.
Includes: Five vials of lyophilized protein

Product Code. 13483139

Quantity Price
1 £ 135.0 / 250µg
Estimated Shipment date
from Supplier 06-12-2016
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Description and Specification

Specification

For Use With (Application) Proteins, Expression, Isolation & Analysis; Enzyme & Protein Activity Assays; Protein Assay Controls, Reference Standards & Accessories
Format Lyophilized
Molecular Weight 34.5kDa
Purity ≥90% by SDS-PAGE
Quantity 5 x 50μg
Storage Requirements Store at -20°C. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles. Expires one year from date of receipt when stored as instructed.
Structural Form Full Length/Recombinant
Protein Subtype EGF (Epidermal Growth Factor)
Endotoxin Concentration <1 EU/μg
Gene Alias AI790464
Common Name Epidermal growth factor
Gene Symbol EGF
Protein Tag IgG1 Fc Tag
Validated Application Assay Standard
Protein Family Growth Factors & Receptors
Expression System Mammalian

Epidermal growth factor (EGF), recombinant mouse protein is supplied as a lyophilized powder. It is suitable for use in performing cell based assays. It can also be used as a protein standard, or in other research applications.
  • Activity: Measured in a cell proliferation assay using BALB/c 3T3 mouse embryonic fibroblasts; the EC50 for this effect is typically 0.2-1ng/mL
  • Formulation: Lyophilized in 140mM NaCl, 2.7mM KCl, 10mM Na2HPO4, 1.8mM KH2PO4, pH 7.4, 5% Mannitol, 5% Trehalose, 0.02% Tween 80
  • Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2mg/mL or lower; aliquot and use as soon as possible

This protein is manufactured by Sino Biological Inc.