Sino Biological™ CDH2 Recombinant Human Protein, His Tag

This recombinant protein was expressed from a DNA sequence encoding the extracellular domain of human CDH2 (NP_001783.2) precursor (Met 1-Ala 724) fused to a polyhistidine tag at the C-terminus

Overview
Brand: Invitrogen

Manufacturer Part Number: 11039H08H250

UNSPSC: 12352202

Code: NEW

Additional Details:
Additional Details: Weight: 0.10000kg



Disclaimers: For Research Use Only. Not for use in diagnostic procedures.

Product Code. 15297605

Quantity Price
1 £ 798.0 / 250µg
Estimated Shipment date
from Supplier 12-05-2017
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Description and Specification

Specification

Storage Requirements Store at -20°C. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles. Expires one year from date of receipt when stored as instructed.
Protein Subtype Intercellular Adhesion Molecule
Validated Application Assay Standard
Molecular Weight 78.5kDa
Expression System Mammalian
Gene Symbol CDH2
Protein Tag His Tag
Gene Alias CD325, CDHN, CDw325, NCAD, CDH2
Common Name CDH2 cadherin 2, type 1, N-cadherin (neuronal)
Protein Family Cell Structure/Organelle Proteins
Purity ≥90% by SDS-PAGE
For Use With (Application) Proteins, Expression, Isolation & Analysis; Enzyme & Protein Activity Assays; Protein Assay Controls, Reference Standards & Accessories
Endotoxin Concentration <1 EU/μg
Format Lyophilized
Quantity 5 x 50μg

Cadherin 2, type 1, N-cadherin (neuronal) (CDH2), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in analysis of protein structure, and performing cell based assays. It can also be used as an immunogen, as a protein standard, or in other research applications.
  • Activity: Measured by the ability of the immobilized protein to support the adhesion of MCF-7 human breast adenocarcinoma cells; when 5 x 104 cells/well are added to recombinant human Cadherin-2 coated plates (0.8μg/mL with 100μL/well), approximately >50% will adhere after 1 hour at 37°C
  • Formulation: Lyophilized in 140mM NaCl, 2.7mM KCl, 10mM Na2HPO4, 1.8mM KH2PO4, pH 7.4, 5% Mannitol, 5% Trehalose, 0.02% Tween 80
  • Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2mg/mL or lower; aliquot and use as soon as possible

This protein is manufactured by Sino Biological Inc.