Sino Biological™ CD86 (B7-2) Recombinant Mouse Protein, His Tag

This recombinant protein was expressed from a DNA sequence encoding the extracellular domain (Met 1-Glu 245) of mouse CD86 precursor (NP_062261.3) fused to a polyhistidine tag at the C-terminus

Brand:  Invitrogen 50068M08H50

Product Code. 13426196

Disclaimers:

For Research Use Only. Not for use in diagnostic procedures.

Description

Cluster of Differentiation 86 (CD86/B7-2), recombinant mouse protein is supplied as a lyophilized powder. It is suitable for use in analysis of protein structure, studying protein-protein interactions, and cell biology research applications. It can also be used as an immunogen, or as a protein standard.

• Activity: Measured by its binding ability in a functional ELISA; immobilized mouse CD86 at 20μg/mL (100μL/well) can bind mouse CD28 with a linear range of 6.4-800ng/mL
• Formulation: Lyophilized in PBS, pH 7.4, 5% Mannitol, 5% Trehalose, and 0.02% Tween 80
• Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2mg/mL or lower; aliquot and use as soon as possible

Shipping Condition: Room Temperature. This protein is manufactured by Sino Biological Inc.

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Specifications

• For Use With (Application): Proteins, Expression, Isolation & Analysis; Enzyme & Protein Activity Assays; Protein Assay Controls, Reference Standards & Accessories
• Gene ID (Entrez): 12524
• Purity: ≥90% by SDS-PAGE
• Storage Requirements: Store at -20° to -70°C; Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles. Expires one year from date of receipt when stored as instructed.
• Protein Subtype: Other Proteins
• Gene Alias: CD86, B7, B7-2, B7.2, B70, CLS1, CD28l2, ETC-1, Ly-58, Ly58, MB7, MB7-2, TS/A-2
• Gene Symbol: CD86
• Protein Family: CD Proteins
• Protein Tag: His Tag

• Format: Lyophilized
• Molecular Weight: 26.8kDa
• Quantity: 50μg
• Structural Form: Truncated/Recombinant/Cell Surface Protein
• Endotoxin Concentration: <1 EU/μg
• Common Name: CD86 antigen
• Validated Application: Assay Standard
• Expression System: Mammalian