Sino Biological™ CD86 (B7-2) Recombinant Human Protein, His Tag

This recombinant protein was expressed from a DNA sequence encoding the extracellular domain (Met 1-His 239) of human CD86 (NP_008820.2) fused to a polyhistidine tag at the C-terminus

Brand:  Invitrogen 10699H08H250

Product Code. 13495936

Disclaimers:

For Research Use Only. Not for use in diagnostic procedures.

Description

Cluster of Differentiation 86 (CD86/B7-2), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in analysis of protein structure, studying protein-protein interactions, and cell biology research applications. It can also be used as an immunogen, or as a protein standard.

• Activity: Measured by its binding ability in a functional ELISA; immobilized human CD86 at 20μg/mL (100μL/well) can bind human CD28 with a linear range of 32-800ng/mL
• Formulation: Lyophilized in PBS, pH 7.4, 5% Mannitol, 5% Trehalose, and 0.02% Tween 80
• Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2mg/mL or lower; aliquot and use as soon as possible

Shipping Condition: Room Temperature. This protein is manufactured by Sino Biological Inc.

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Specifications

• For Use With (Application): Proteins, Expression, Isolation & Analysis; Enzyme & Protein Activity Assays; Protein Assay Controls, Reference Standards & Accessories
• Gene ID (Entrez): 942
• Purity: ≥90% by SDS-PAGE
• Storage Requirements: Store at -20° to -70°C. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles. Expires one year from date of receipt when stored as instructed.
• Protein Subtype: Other Proteins
• Gene Alias: B7-2, B70, CD28LG2, LAB72, mgC34413
• Gene Symbol: CD86
• Protein Family: CD Proteins
• Protein Tag: His Tag

• Format: Lyophilized
• Molecular Weight: 26.2kDa
• Quantity: 5 x 50μg
• Structural Form: Truncated/Recombinant/Cell Surface Protein
• Endotoxin Concentration: <1 EU/μg
• Common Name: CD86 molecule
• Validated Application: Assay Standard
• Expression System: Mammalian