Sino Biological™ CD4 Recombinant Mouse Protein, His Tag

This recombinant protein was expressed from a DNA sequence encoding the extracellular domain (Met 1-Thr 394) of mouse CD4 (NP_038516.1) fused to a polyhistidine tag at the C-terminus

Overview
Brand: Invitrogen

Manufacturer Part Number: 50134M08H5

5UG RECOM MS CD4 (HIS) ACTIVE

Code: NEW

Additional Details:
Additional Details: Weight: 0.01000kg



Disclaimers: For Research Use Only. Not for use in diagnostic procedures.

Product Code. 15342796

Quantity Price
1 £ 54.25 / 5µg
Estimated Shipment date
from Supplier 13-12-2016
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Description and Specification

Specification

For Use With (Application) Proteins, Expression, Isolation & Analysis; Enzyme & Protein Activity Assays; Protein Assay Controls, Reference Standards & Accessories
Format Lyophilized
Gene ID (Entrez) 12504
Molecular Weight 42.8kDa
Purity ≥95% by SDS-PAGE
Quantity 5μg
Storage Requirements Store at -20° to -70°C. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles. Expires one year from date of receipt when stored as instructed.
Structural Form Truncated/Recombinant/Cell Surface Protein
Protein Subtype Glycoproteins
Endotoxin Concentration <1 EU/μg
Gene Alias L3T4, Ly-4, CD4
Common Name CD4 antigen
Gene Symbol CD4
Protein Tag His Tag
Validated Application Assay Standard
Protein Family CD Proteins
Expression System Mammalian

Cluster of Differentiation 4 (CD4), recombinant mouse protein is supplied as a lyophilized powder. It is suitable for use in analysis of protein structure, and cell biology research applications. It can also be used as an immunogen, or as a protein standard.
  • Activity: Measured by the ability of the immobilized protein to support the adhesion of HeLa human cervical epithelial carcinoma cells; when 5 x 104 cells/well are added to CD4 coated plates (2.5μg/mL and 100μL/well), approximately 40-60 % will adhere specifically after 60 minutes at 37°C
  • Formulation: Lyophilized in 140mM NaCl, 2.7mM KCl, 10mM Na2HPO4, 1.8mM KH2PO4, pH 7.4, 5% Mannitol, 5% Trehalose, 0.02% Tween 80
  • Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2mg/mL or lower; aliquot and use as soon as possible

This protein is manufactured by Sino Biological Inc.