Sino Biological™ CD4 Recombinant Human Protein, His Tag

This recombinant protein was expressed from a DNA sequence encoding the extracellular domain (Met 1-Trp 390) of human CD4 (NP_000607.1) fused to a polyhistidine tag at the C-terminus

Overview
Brand: Invitrogen

Manufacturer Part Number: 10400H08H5

UNSPSC: 12352202

Code: NEW

Additional Details:
Additional Details: Weight: 0.01000kg



Disclaimers: For Research Use Only. Not for use in diagnostic procedures.

Product Code. 13403019

Quantity Price
1 £ 53.25 / 5µg
Estimated Shipment date
from Supplier 10-04-2017
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Description and Specification

Specification

Quantity 5μg
Storage Requirements Store at -20° to -70°C. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles. Expires one year from date of receipt when stored as instructed.
Gene Symbol CD4
Molecular Weight 42.2kDa
Protein Family CD Proteins
Gene ID (Entrez) 920
For Use With (Application) Proteins, Expression, Isolation & Analysis; Enzyme & Protein Activity Assays; Protein Assay Controls, Reference Standards & Accessories
Format Lyophilized
Structural Form Truncated/Recombinant/Cell Surface Protein
Protein Subtype Glycoproteins
Common Name CD4 molecule
Expression System Mammalian
Validated Application Assay Standard
Gene Alias CD4mut
Purity ≥90% by SDS-PAGE
Protein Tag His Tag
Endotoxin Concentration <1 EU/μg

Cluster of Differentiation 4 (CD4), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in analysis of protein structure, studying protein-protein interactions, and cell biology research applications. It can also be used as an immunogen, or as a protein standard.
  • Activity: 1. Measured by its ability to bind to HIV-1 gp120 in a functional ELISA; 2. Measured by the ability of the immobilized protein to support the adhesion of HeLa human cervical epithelial carcinoma cells; when 5 x 104 cells/well are added to CD4 coated plates (2.5μg/mL and 100μL/well), approximately 60-80% will adhere specifically after 60 minutes at 37°C
  • Formulation: Lyophilized in PBS, pH 7.4, 5% Mannitol, 5% Trehalose, and 0.02% Tween 80
  • Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2mg/mL or lower; aliquot and use as soon as possible

This protein is manufactured by Sino Biological Inc.