Sino Biological™ CD28 Recombinant Mouse Protein, hIgG1-Fc.His Tag

This recombinant protein was expressed from a DNA sequence encoding the extracellular domain (Met 1-Lys 149) of mouse CD28 precursor (NP_031668.3) fused to the polyhistidine-tagged Fc region of human IgG1 at the C-terminus

Brand: Invitrogen

Manufacturer Part Number: 50103M03H25

CD28 Recombinant Mouse Protein (hIgG1-Fc.His Tag,

UNSPSC: 12352202

Code: NEW

Additional Details:
Additional Details: Weight: 0.01000kg

Disclaimers: For Research Use Only. Not for use in diagnostic procedures.

Product Code. 13416226

Quantity Price
1 £135.00 / 25µg
Estimated Shipment date
from Supplier 25-10-2016
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Description and Specification


For Use With (Application) Proteins, Expression, Isolation & Analysis; Enzyme & Protein Activity Assays; Protein Assay Controls, Reference Standards & Accessories
Format Lyophilized
Gene ID (Entrez) 12487
Molecular Weight 43kDa
Purity ≥90% by SDS-PAGE
Quantity 5 x 5μg
Storage Requirements Store at -20° to -70°C. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles. Expires one year from date of receipt when stored as instructed.
Structural Form Truncated/Recombinant/Cell Surface Protein
Protein Subtype Glycoproteins
Endotoxin Concentration <1 EU/μg
Gene Alias CD28, CH29-189K17.3
Common Name CD28 antigen
Gene Symbol CD28
Protein Tag His Tag/IgG1 Fc Tag
Validated Application Assay Standard
Protein Family CD Proteins
Expression System Mammalian

Cluster of Differentiation 28 (CD28), recombinant mouse protein is supplied as a lyophilized powder. It is suitable for use in studying protein-protein interactions, and cell biology research applications. It can also be used as a protein standard.
  • Activity: Measured by its binding ability in a functional ELISA; 1. Immobilized mouse CD86 at 20μg/mL (100μL/well) can bind mouse CD28 -Fc with a linear range of 6.4-800ng/mL; 2. Immobilized rat CD86 at 2μg/mL (100μL/well) can bind mouse CD28-Fc with a linear range of 6.4-160ng/mL
  • Formulation: Lyophilized in PBS, pH 7.4, 5% Mannitol, 5% Trehalose, and 0.02% Tween 80
  • Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2mg/mL or lower; aliquot and use as soon as possible

Shipping Condition: Room Temperature. This protein is manufactured by Sino Biological Inc.