Sino Biological™ CD274 Recombinant Mouse Protein, hIgG1-Fc.His Tag

This recombinant protein was expressed from a DNA sequence encoding the extracellular domain (Met 1-Thr 238) of mouse CD274 (NP_068693.1) fused to the C-terminal His-tagged Fc region of human IgG1 at the C-terminus

Overview
Brand: Invitrogen

Manufacturer Part Number: 50010M03H50

50UG RECOM MS CD274 (HIGG1-FC-HIS)

Code: NEW

Additional Details:
Additional Details: Weight: 0.01000kg



Disclaimers: For Research Use Only. Not for use in diagnostic procedures.

Product Code. 15342526

Quantity Price
1 £ 158.0 / 50µg
Estimated Shipment date
from Supplier 07-12-2016
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Description and Specification

Specification

For Use With (Application) Proteins, Expression, Isolation & Analysis; Enzyme & Protein Activity Assays; Protein Assay Controls, Reference Standards & Accessories
Format Lyophilized
Molecular Weight 52.8kDa
Purity ≥90% by SDS-PAGE
Quantity 50μg
Storage Requirements Store at -20°C. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles.Expires one year from date of receipt when stored as instructed.
Protein Subtype Other Proteins
Endotoxin Concentration <1 EU/μg
Gene Alias CD274, B7-H1, PD-L1, Pdcd1l1, Pdcd1lg1
Common Name CD274 Antigen
Gene Symbol CD274
Protein Tag His Tag/IgG1 Fc Tag
Validated Application Assay Standard
Protein Family CD Proteins
Expression System Mammalian

CD274 Antigen (CD274), recombinant mouse protein is supplied as a lyophilized powder. It is suitable for use in protein studies such as protein structure analysis and protein-protein interactions. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications.
  • Activity: Measured by its binding ability in a functional ELISA; immobilized recombinant mouse PD-1 at 10μg/mL (100μL/well) can bind mouse CD274 with a linear range of 3.2-400ng/mL
  • Formulation: Lyophilized in 140mM NaCl, 2.7mM KCl, 10mM Na2HPO4, 1.8mM KH2PO4, pH 7.4, 5% Mannitol, 5% Trehalose, 0.02% Tween 80
  • Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2mg/mL or lower; aliquot and use as soon as possible

This protein is manufactured by Sino Biological Inc.