Sino Biological™ CD19 Recombinant Human Protein, His Tag

This recombinant protein is expressed from a DNA sequence encoding the extracellular domain (Met 1-Lys 291) of human CD19 (AAB60697) fused to a polyhistidine tag at the C-terminus

Overview
Brand: Invitrogen

Manufacturer Part Number: 11880H08H5

UNSPSC: 12352202

Code: NEW

Additional Details:
Additional Details: Weight: 0.01000kg



Disclaimers: For Research Use Only. Not for use in diagnostic procedures.

Product Code. 13415606

Quantity Price
1 £ 97.5 / 5µg
EU Stock 3
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Description and Specification

Specification

Purity ≥90% by SDS-PAGE
Structural Form Truncated/Recombinant/Cell Surface Protein
Molecular Weight 31.6kDa
Protein Tag His Tag
Storage Requirements Store at -20° to -70°C. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles. Expires one year from date of receipt when stored as instructed.
Gene ID (Entrez) 930
Format Lyophilized
Gene Alias B4, mgC12802
Gene Symbol CD19
Validated Application Assay Standard
For Use With (Application) Proteins, Expression, Isolation & Analysis; Enzyme & Protein Activity Assays; Protein Assay Controls, Reference Standards & Accessories
Protein Family CD Proteins
Endotoxin Concentration <1 EU/μg
Expression System Mammalian
Quantity 5μg
Common Name CD19 molecule
Protein Subtype Other Proteins

Cluster of Differentiation 19 (CD19) recombinant human protein is supplied as a lyophilized powder. This protein is suitable for use in analysis of protein structure the study of protein-protein interactions, and cell biology research applications. It can also be used as an immunogen, or as a protein standard.&line-break;Background: Cluster of Differentiation 19 (CD19), is a single-pass type I membrane protein which contains two Ig-like C2-type (immunoglobulinlike) domains. CD19 forms a complex with CD21, CD81 and CD225 in the membrane of mature B cells. It interacts with VAV. It interacts with GRB2 and SOS when phosphorylated on Tyr-348 and/or Tyr-378. CD19 also interacts with PLCG2 when phosphorylated on Tyr-409. CD19 is an important pan B cell marker and co-stimulatory protein in humans and mice. CD19 is a hallmark differentiation antigen of the B cell lineage and positively regulates antigen receptor signal transduction in mature B cells. CD19 defines signaling thresholds for the Ag receptor as well as other cell surface receptors that regulate B lymphocyte selection, activation, and differentiation. Defects in CD19 are a cause of hypogammaglobulinemia.
  • N-terminal Sequence Analysis: Pro 20
  • Activity: Measured by its binding ability in a functional ELISA; immobilized human CD19 at 2μg/mL (100μL/well) has been shown to bind biotinylated human CD9 with a linear range of 0.032-0.8μg/mL
  • Formulation: lyophilized in PBS, pH 7.4, 5% mannitol, 5% trehalose, and 0.02% Tween®-80
  • Reconstitution: Dissolve the protein in sterile double-distilled water to a concentration of 0.2mg/mL or lower; aliquot and use as soon as possible

Shipping Condition: Room Temperature. Manufactured by Sino Biological (China).