Sino Biological™ BACE1 Recombinant Mouse Protein, His Tag

This recombinant protein was expressed from a DNA sequence encoding the extracellular domain (Met 1-Thr 457) of the mouse BACE1 precursor (NM_011792.5) fused to the polyhistidine tag at the C-terminus

Overview
Brand: Invitrogen

Manufacturer Part Number: 50002M08H25

25UG RECOM MS BACE1 (HIS)

Code: NEW

Additional Details:
Additional Details: Weight: 0.01000kg



Disclaimers: For Research Use Only. Not for use in diagnostic procedures.

Product Code. 15372506

Quantity Price
1 £ 122.0 / 25µg
Estimated Shipment date
from Supplier 07-12-2016
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Description and Specification

Specification

For Use With (Application) Proteins, Expression, Isolation & Analysis; Enzyme & Protein Activity Assays; Protein Assay Controls, Reference Standards & Accessories
Format Lyophilized
Molecular Weight 49.8kDa
Purity ≥90% by SDS-PAGE
Quantity 5 x 5μg
Storage Requirements Store at -20°C. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles. Expires one year from date of receipt when stored as instructed.
Protein Subtype Proteases
Endotoxin Concentration <1 EU/μg
Gene Alias Bace1, C76936
Common Name beta-site APP cleaving enzyme 1
Gene Symbol BACE1
Protein Tag His Tag
Validated Application Assay Standard
Protein Family Other Proteins
Expression System Mammalian

Beta-site APP-cleaving enzyme 1 (BACE1), recombinant mouse protein is supplied as a lyophilized powder. It is suitable for use in analysis of protein structure. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications.
  • Activity: Measured by its ability to cleave a fluorescent peptide substrate Mca-Ser-Glu-Val-Asn-Leu-Asp-Ala-Glu-Phe-Arg-Lys(Dpn)-Arg-Arg-NH2, cleavage of this substrate can be measured using excitation and emission wavelengths of 320 and 405nM, respectively; the specific activity is >2pmole/min./μg
  • Formulation: Lyophilized in 140mM NaCl, 2.7mM KCl, 10mM Na2HPO4, 1.8mM KH2PO4, pH 7.4, 5% Mannitol, 5% Trehalose, 0.02% Tween 80
  • Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2mg/mL or lower; aliquot and use as soon as possible

This protein is manufactured by Sino Biological Inc.