Siglec H Rat anti-Mouse, PE-Cy7, Clone: eBio440c, eBioscience™

Rat Monoclonal Antibody

Overview
Brand: Affymetrix eBioscience

Manufacturer Part Number: 25-0333-82

Code: NEW

Additional Details:
Additional Details: Weight: 0.09500kg



Disclaimers: For Research Use Only.

Product Code. 15568386

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Description and Specification

Specification

Quantity 100μg
Storage Requirements Store at 2-8°C. Do not freeze. Light-sensitive material. This tandem dye is sensitive to photo-induced oxidation. Protect this vial from light during storage
Applications Flow Cytometry
Clone eBio440c
Formulation aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
Isotype IgG2b
Antigen Siglec H
Gene Alias Sialic Acid-Binding Immunoglobulin-Like Lectin H
Concentration 0.2mg/mL
Conjugate PE-Cy7
Monoclonal or Polyclonal Monoclonal
Regulatory Status RUO
Format Conjugated
Species Reactivity Mouse
Primary or Secondary Primary
Host Species Rat

The monoclonal antibody eBio440c recognizes Siglec-H, a protein exclusively found on pDC (plasmacytoid dendritic cells) or type I IFN-producing cells (IPC) in the naïve mouse. Mouse IPC are typically PDCA+, CD11c+, CD11b-, B220+, and Ly-6C+, and are quick to respond to viruses. Siglec-H is a transmembrane protein of the Ig superfamily that like CD33 have been shown to bind sialic acid but lacks the characteristic cytoplasmic ITIM domain (immunoreceptor tyrosine based inhibitory motif). To overcome the lack of a cytoplasmic domain, Siglec-H associates with DAP12 thereby allowing for signal transduction.

The eBio440c antibody has been shown to inhibit pDC function (inhbits IFN alpha secretion in response to CpG).

It has been observed that some mouse strains (such as SJL) have higher percentages of pDCs compared to C57BL/6.