Siglec H Rat anti-Mouse, PE-Cy7, Clone: eBio440c, eBioscience™

Rat Monoclonal Antibody

Overview
Brand: Affymetrix eBioscience

Manufacturer Part Number: 25-0333-82

100UG Anti-Mouse Siglec H PE-Cyanine7

Code: NEW

Additional Details:
Additional Details: Weight: 0.09500kg



Disclaimers: For Research Use Only.

Product Code. 15568386

Quantity Price
1 £ 278.6 / 100µg
Estimated Shipment date
from Supplier 20-02-2017
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Description and Specification

Specification

Antigen Siglec H
Formulation aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
Gene Alias Sialic Acid-Binding Immunoglobulin-Like Lectin H
Applications Flow Cytometry
Isotype IgG2b
Quantity 100μg
Regulatory Status RUO
Storage Requirements Store at 2-8°C. Do not freeze. Light-sensitive material. This tandem dye is sensitive to photo-induced oxidation. Protect this vial from light during storage
Monoclonal or Polyclonal Monoclonal
Clone eBio440c
Concentration 0.2mg/mL
Conjugate PE-Cy7
Format Conjugated
Host Species Rat
Species Reactivity Mouse
Primary or Secondary Primary

The monoclonal antibody eBio440c recognizes Siglec-H, a protein exclusively found on pDC (plasmacytoid dendritic cells) or type I IFN-producing cells (IPC) in the naïve mouse. Mouse IPC are typically PDCA+, CD11c+, CD11b-, B220+, and Ly-6C+, and are quick to respond to viruses. Siglec-H is a transmembrane protein of the Ig superfamily that like CD33 have been shown to bind sialic acid but lacks the characteristic cytoplasmic ITIM domain (immunoreceptor tyrosine based inhibitory motif). To overcome the lack of a cytoplasmic domain, Siglec-H associates with DAP12 thereby allowing for signal transduction.

The eBio440c antibody has been shown to inhibit pDC function (inhbits IFN alpha secretion in response to CpG).

It has been observed that some mouse strains (such as SJL) have higher percentages of pDCs compared to C57BL/6.