siglec H Rat anti-Mouse, eFluor 660, Clone: eBio440c, eBioscience™

Rat Monoclonal Antibody

Overview
Brand: Affymetrix eBioscience

Manufacturer Part Number: 50-0333-82

UNSPSC: 12352200

Code: Z2

Additional Details:
Additional Details: Weight: 0.25000kg



Disclaimers: For Research Use Only.

Product Code. 15361860

Quantity Price
1 £ 170.59 / 100µg
Estimated Shipment date
from Supplier 04-05-2017
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Description and Specification

Specification

Formulation aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
Applications Flow Cytometry
Antigen siglec H
Storage Requirements Store at 2-8°C. Do not freeze. Light-sensitive material.
Primary or Secondary Primary
Monoclonal or Polyclonal Monoclonal
Conjugate eFluor 660
Quantity 100μg
Species Reactivity Mouse
Gene Alias Sialic Acid-Binding Immunoglobulin-Like Lectin H
Clone eBio440c
Format Conjugated
Host Species Rat
Concentration 0.2mg/mL
Regulatory Status RUO
Isotype IgG2b

The monoclonal antibody eBio440c recognizes Siglec-H, a protein exclusively found on pDC (plasmacytoid dendritic cells) or type I IFN-producing cells (IPC) in the naïve mouse. Mouse IPC are typically PDCA+, CD11c+, CD11b-, B220+, and Ly-6C+, and are quick to respond to viruses. Siglec-H is a transmembrane protein of the Ig superfamily that like CD33 have been shown to bind sialic acid but lacks the characteristic cytoplasmic ITIM domain (immunoreceptor tyrosine based inhibitory motif). To overcome the lack of a cytoplasmic domain, Siglec-H associates with DAP12 thereby allowing for signal transduction.

The eBio440c antibody has been shown to inhibit pDC function (inhbits IFNalpha secretion in response to CpG).

It has been observed that some mouse strains (such as SJL) have higher percentages of pDCs compared to C57BL/6.