siglec H Rat anti-Mouse, eFluor 450, Clone: eBio440c, eBioscience™

Rat Monoclonal Antibody

Brand: Affymetrix eBioscience

Manufacturer Part Number: 48-0333-80

25UG Anti-Mouse Siglec H eFluor 450

Code: NEW

Additional Details:
Additional Details: Weight: 0.09500kg

Disclaimers: For Research Use Only.

Product Code. 15509666

Quantity Price
1 £116.50 / 25µg
Estimated Shipment date
from Supplier 26-10-2016
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Description and Specification


Antigen siglec H
Applications Flow Cytometry
Clone eBio440c
Concentration 0.2mg/mL
Conjugate eFluor 450
Format Conjugated
Formulation aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
Gene Alias Sialic Acid-Binding Immunoglobulin-Like Lectin H
Host Species Rat
Isotype IgG2b
Quantity 25μg
Regulatory Status RUO
Species Reactivity Mouse
Storage Requirements Store at 2-8°C. Do not freeze. Light-sensitive material.
Primary or Secondary Primary
Monoclonal or Polyclonal Monoclonal

The monoclonal antibody eBio440c recognizes Siglec-H, a protein exclusively found on pDC (plasmacytoid dendritic cells) or type I IFN-producing cells (IPC) in the naïve mouse. Mouse IPC are typically PDCA+, CD11c+, CD11b-, B220+, and Ly-6C+, and are quick to respond to viruses. Siglec-H is a transmembrane protein of the Ig superfamily that like CD33 have been shown to bind sialic acid but lacks the characteristic cytoplasmic ITIM domain (immunoreceptor tyrosine based inhibitory motif). To overcome the lack of a cytoplasmic domain, Siglec-H associates with DAP12 thereby allowing for signal transduction.

The eBio440c antibody has been shown to inhibit pDC function (inhbits IFNalpha secretion in response to CpG).

It has been observed that some mouse strains (such as SJL) have higher percentages of pDCs compared to C57BL/6.