siglec H Rat anti-Mouse, eFluor 450, Clone: eBio440c, eBioscience™

Rat Monoclonal Antibody

Overview
Brand: Affymetrix eBioscience

Manufacturer Part Number: 48-0333-80

Code: NEW

Additional Details:
Additional Details: Weight: 0.09500kg



Disclaimers: For Research Use Only.

Product Code. 15509666

Quantity Price
1 £ 76.43 / 25µg
Estimated Shipment date
from Supplier 04-05-2017
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Description and Specification

Specification

Gene Alias Sialic Acid-Binding Immunoglobulin-Like Lectin H
Quantity 25μg
Concentration 0.2mg/mL
Host Species Rat
Primary or Secondary Primary
Monoclonal or Polyclonal Monoclonal
Format Conjugated
Storage Requirements Store at 2-8°C. Do not freeze. Light-sensitive material.
Antigen siglec H
Applications Flow Cytometry
Formulation aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
Species Reactivity Mouse
Isotype IgG2b
Conjugate eFluor 450
Regulatory Status RUO
Clone eBio440c

The monoclonal antibody eBio440c recognizes Siglec-H, a protein exclusively found on pDC (plasmacytoid dendritic cells) or type I IFN-producing cells (IPC) in the naïve mouse. Mouse IPC are typically PDCA+, CD11c+, CD11b-, B220+, and Ly-6C+, and are quick to respond to viruses. Siglec-H is a transmembrane protein of the Ig superfamily that like CD33 have been shown to bind sialic acid but lacks the characteristic cytoplasmic ITIM domain (immunoreceptor tyrosine based inhibitory motif). To overcome the lack of a cytoplasmic domain, Siglec-H associates with DAP12 thereby allowing for signal transduction.

The eBio440c antibody has been shown to inhibit pDC function (inhbits IFNalpha secretion in response to CpG).

It has been observed that some mouse strains (such as SJL) have higher percentages of pDCs compared to C57BL/6.