siglec H Rat anti-Mouse, eFluor 450, Clone: eBio440c, eBioscience™

Rat Monoclonal Antibody

Overview
Brand: Affymetrix eBioscience

Manufacturer Part Number: 48-0333-82

Code: NEW

Additional Details:
Additional Details: Weight: 0.09500kg



Disclaimers: For Research Use Only.

Product Code. 15519666

Quantity Price
1 £ 188.93 / 100µg
Estimated Shipment date
from Supplier 05-05-2017
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Description and Specification

Specification

Concentration 0.2mg/mL
Primary or Secondary Primary
Storage Requirements Store at 2-8°C. Do not freeze. Light-sensitive material.
Format Conjugated
Applications Flow Cytometry
Gene Alias Sialic Acid-Binding Immunoglobulin-Like Lectin H
Host Species Rat
Isotype IgG2b
Species Reactivity Mouse
Antigen siglec H
Formulation aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
Monoclonal or Polyclonal Monoclonal
Clone eBio440c
Conjugate eFluor 450
Quantity 100μg
Regulatory Status RUO

The monoclonal antibody eBio440c recognizes Siglec-H, a protein exclusively found on pDC (plasmacytoid dendritic cells) or type I IFN-producing cells (IPC) in the naïve mouse. Mouse IPC are typically PDCA+, CD11c+, CD11b-, B220+, and Ly-6C+, and are quick to respond to viruses. Siglec-H is a transmembrane protein of the Ig superfamily that like CD33 have been shown to bind sialic acid but lacks the characteristic cytoplasmic ITIM domain (immunoreceptor tyrosine based inhibitory motif). To overcome the lack of a cytoplasmic domain, Siglec-H associates with DAP12 thereby allowing for signal transduction.

The eBio440c antibody has been shown to inhibit pDC function (inhbits IFNalpha secretion in response to CpG).

It has been observed that some mouse strains (such as SJL) have higher percentages of pDCs compared to C57BL/6.