missing translation for 'onlineSavingsMsg'
Learn More

Siglec-G, PerCP-eFluor™ 710, clone: SH2.1, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 46-5833-80

Code : NEW

Additional Details : Weight : 0.09500kg

 View more versions of this product

Product Code. 15529286

  • £83.94 / 25µg

Estimated Shipment date
from Supplier 26-06-2018
Add to basket



Description: The SH2.1 monoclonal antibody reacts with mouse siglec-G, which is also known as sialic acid-binding immunoglobulin-like lectin-G. Siglec-G is an inhibitory receptor that is expressed in a B cell-specific manner with B1 B cells expressing the highest levels of siglec-G. When overexpressed, siglec-G inhibits B cell receptor-mediated calcium signaling. Siglec-G-deficient mice show a massive expansion of the B1a cell population as well as higher titers of natural IgM antibodies. Siglec-G-deficient B1a cells show a lower level of spontaneous apoptosis and a prolonged life span, which may result from higher expression levels of the transcription factor NFATc1. Siglec-G-dependent negative regulation exists in B1 cells, which may explain the naturally muted signaling response of B1 cells.Applications Reported: This SH2.1 antibody has been reported for use in flow cytometric analysis.Applications Tested: This SH2.1 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.125 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5. We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative. Please make sure that your instrument is capable of detecting this fluorochrome.Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 μL cell sample + 100 μL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.Filtration: 0.2μm post-manufacturing filtered.


Siglec G
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
IgG1, kappa
Store at 2-8°C. Do not freeze. Light-sensitive material.
Flow Cytometry
PerCP-eFluor 710
Sialic Acid-Binding Immunoglobulin-Like Lectin-G, SiglecG, Siglec10, Siglec 10
Affinity chromatography

For Research Use Only.