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Siglec-G, APC, clone: SH2.1, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 17-5833-82

Code : NEW

Additional Details : Weight : 0.09500kg

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Product Code. 15518146

  • £223.65 / 100µg


EU Stock 5
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Description

Description

Description: The SH2.1 monoclonal antibody reacts with mouse siglec-G, which is also known as sialic acid-binding immunoglobulin-like lectin-G. Siglec-G is an inhibitory receptor that is expressed in a B cell-specific manner with B1 B cells expressing the highest levels of siglec-G. When overexpressed, siglec-G inhibits B cell receptor-mediated calcium signaling. Siglec-G-deficient mice show a massive expansion of the B1a cell population as well as higher titers of natural IgM antibodies. Siglec-G-deficient B1a cells show a lower level of spontaneous apoptosis and a prolonged life span, which may result from higher expression levels of the transcription factor NFATc1. Siglec-G-dependent negative regulation exists in B1 cells, which may explain the naturally muted signaling response of B1 cells.Applications Reported: This SH2.1 antibody has been reported for use in flow cytometric analysis.Applications Tested: This SH2.1 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.125 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

Siglec G
Monoclonal
0.2mg/mL
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
Conjugated
Siglecg
IgG1, kappa
100μg
Store at 2-8°C. Do not freeze. Light-sensitive material.
Primary
243958
Flow Cytometry
SH2.1
APC
Siglecg
Sialic Acid-Binding Immunoglobulin-Like Lectin-G, SiglecG, Siglec10, Siglec 10
Murine
Affinity chromatography
RUO
Antibody
Monoclonal
Murine
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