The SequaGel™ sequencing system consists of SequaGel™ Concentrate, SequaGel™ Diluent, and SequaGel™ Buffer. This system provides a convenient, dependable means for researchers to prepare gels of varying percentage. With the SequaGel™ sequencing system the researcher readily prepares any commonly used gel formulation up to 20% monomer (19:1 Acrylamide: Bis-acrylamide). 1L bottle of SequaGel™ Concentrate contains 237.5g of acrylamide, 12. 5g of methylene bisacrylamide, and urea in a deionised aqueous solution. SequaGel™ Diluent is supplied in 450mL and 1L bottle containing urea in deionised water. SequaGel™ Buffer is supplied in 100mL and 200mL bottles containing 0.89M Tris-Borate-20mM EDTA buffer pH8.3 (10X TBE) and urea.
Casts 19:1 denaturing gels up to 20% monomer
Aldehyde free and ammonia free
Stabilised for long shelf life
Consistently crystal clear gels For DNA sequencing, manual.