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SEMA4A, PerCP-eFluor™ 710, clone: 5E3, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 46-9753-41

Code : NEW

Additional Details : Weight : 0.23750kg

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Product Code. 15519436

  • £87.94 / 25 tests


Estimated Shipment date
from Supplier 01-06-2018
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Description

Description

Description: The monoclonal antibody 5E3 reacts with human and mouse Semaphorin 4A (Sema4A). This 150kDa glycoprotein is a member of the class IV semaphorin family and exists as membrane-bound and soluble forms. Sema4A is expressed by a wide variety of tissues, including lung, kidney, spleen, testis, and mammary glands, as well as activated T cells and dendritic cells. This ligand binds to Tim-2, neuropilin-1 (Nrp1), plexin D1, and plexin B1. Sema4A plays a role in T cell activation, Th1 differentiation, and Treg function and survival.Our testing demonstrates that the 5E3 antibody stains resting CD49b+ NK cells in mouse. However, staining of CD56+ NK cells in human is negative.Applications Reported: This 5E3 antibody has been reported for use in flow cytometric analysis.Applications Tested: This 5E3 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5μL (0.06μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5. We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative. Please make sure that your instrument is capable of detecting this fluorochrome.Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100μL of cell sample + 100μL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

Semaphorin 4A
Monoclonal
5μl/Test
PBS with 0.1% gelatin, 0.2% BSA and 0.09% sodium azide; pH 7.2
Q9H3S1, Q62178
CORD10, RP35, SEMAB, SEMB, sema B, semaphorin-4A, semaphorin-B
Murine
Affinity chromatography
RUO
Antibody
Monoclonal
Murine
Flow Cytometry
5.00E+03
PerCP-eFluor 710
SEMA4A
Conjugated
SEMA4A
IgG1, kappa
25 Tests
Store at 2-8°C. Do not freeze. Light-sensitive material. This tandem dye is sensitive to photo-induced oxidation. Protect this vial from light during storage, handling and experimental procedures.
Primary
64218
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