Rapid purification of His-tagged recombinant Proteins from cell lysates
UF-concentrato rs and Buffers
MiniprepMC spin column Kit
Vivapure maxiprep MC spin columns are centrifugal devices for rapid purification of His-tagged recombinant proteins from cell lysates by metal chelate affinity chromatography. The pre-packed spin columns incorporate Ni2+-IDA agarose resin plugs as the chromatographic matrix. The rapid 1-2-3 bind-wash-elute protocol especially lends itself to screening applications, where many samples are processed in parallel. Vivapure maxiprep MC spin columns can conveniently be used in a centrifuge with swing-out rotor for standard 50 ml tubes. Binding capacity is about 10 mg His-tagged protein per unit. The low hold-up volume ensures high solute recovery with minimal loss by non-specific adsorption. Vivapure maxiprep MC spin columns can be re-used twice and are available as complete kits including buffers or as bulk packs.They are an attractive alternative to lengthy chromatographic methods such as FPLC. For processing larger sample volumes than 20 ml, e. g. from diluted cell culture supernatants, the Vivapure maxiprep spin columns can additionally be used with a sealing cap and pump collar for peristaltic pumps.