RUNX3 Mouse anti-Human, eFluor™ 660, Clone: R3-5G4, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 50-9817-82

Product Code. 15530147

Description

Description: This R3-5G4 monoclonal antibody recognizes human Runx3, a 44 kDa transcription factor that belongs to the Runt domain-containing family. Runx3 is expressed in hematopoietic cells such as T cells, B cells, and myeloid cells. This transcription factor is also expressed in non-hematopoietic tissues, including the epidermis, bone, and dorsal root ganglia. Runx3 has been established to play a critical role in thymocyte development. More specifically, Runx3 promotes CD8 + T cell development by silencing Cd4 expression. Finally, studies have demonstrated the tumor suppressive activity of in a variety of cancers (e.g., gastric and breast cancers). Applications Reported: This R3-5G4 antibody has been reported for use in immunohistochemical staining. Applications Tested: This R3-5G4 antibody has been tested by immunohistochemistry on FFPE human tissue using high pH antigen retrieval. eFluor™ 660 is a replacement for Alexa Fluor™ 647. eFluor™ 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.The antibody can be used at less than or equal to 10 μg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser. Filtration: 0.2 μm post-manufacturing filtered.

This gene encodes a member of the runt domain-containing family of transcription factors. A heterodimer of this protein and a beta subunit forms a complex that binds to the core DNA sequence 5′-PYGPYGGT-3′ found in a number of enhancers and promoters, and can either activate or suppress transcription. It also interacts with other transcription factors. It functions as a tumor suppressor, and the gene is frequently deleted or transcriptionally silenced in cancer. Multiple transcript variants encoding different isoforms have been found for this gene.

Specifications

• Antigen: Runx3
• Classification: Monoclonal
• Concentration: 0.2 mg/mL
• Formulation: PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
• Gene Accession No.: Q13761
• Host Species: Mouse
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG1 κ

• Applications: Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin)
• Clone: R3-5G4
• Conjugate: eFluor 660
• Gene: RUNX3
• Gene Symbols: RUNX3
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 864
• Content And Storage: 4° C, store in dark, DO NOT FREEZE!
• Form: Liquid

For Research Use Only.