RUNX3 Mouse anti-Human, Biotin, Clone: R3-5G4, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 13-9817-82

Product Code. 15517396

Description

Description: This R3-5G4 monoclonal antibody recognizes human Runx3, a 44 kDa transcription factor that belongs to the Runt domain-containing family. Runx3 is expressed in hematopoietic cells such as T cells, B cells, and myeloid cells. This transcription factor is also expressed in non-hematopoietic tissues, including the epidermis, bone, and dorsal root ganglia. Runx3 has been established to play a critical role in thymocyte development. More specifically, Runx3 promotes CD8 + T cell development by silencing Cd4 expression. Finally, studies have demonstrated the tumor suppressive activity of in a variety of cancers (e.g., gastric and breast cancers). Applications Reported: This R3-5G4 antibody has been reported for use in western blotting, and immunohistochemical staining of formalin-fixed paraffin embedded tissue sections. Applications Tested: This R3-5G4 antibody has been tested by immunohistochemistry on human FFPE tissue sections using high pH antigen retrieval at less than or equal to 20 μg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Filtration: 0.2 μm post-manufacturing filtered.

This gene encodes a member of the runt domain-containing family of transcription factors. A heterodimer of this protein and a beta subunit forms a complex that binds to the core DNA sequence 5′-PYGPYGGT-3′ found in a number of enhancers and promoters, and can either activate or suppress transcription. It also interacts with other transcription factors. It functions as a tumor suppressor, and the gene is frequently deleted or transcriptionally silenced in cancer. Multiple transcript variants encoding different isoforms have been found for this gene.

Specifications

• Antigen: Runx3
• Classification: Monoclonal
• Concentration: 0.5 mg/mL
• Formulation: PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
• Gene Accession No.: Q13761
• Host Species: Mouse
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG1 κ

• Applications: Immunohistochemistry (Paraffin), Western Blot
• Clone: R3-5G4
• Conjugate: Biotin
• Gene: RUNX3
• Gene Symbols: RUNX3
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 864
• Content And Storage: 4° C, store in dark, DO NOT FREEZE!
• Form: Liquid

For Research Use Only.