ROR gamma (t), clone: AFKJS-9, eBioscience™

Rat Monoclonal Antibody

Brand:  Affymetrix eBioscience 14-6988-80

Product Code. 15267957

Description

Description: The AFKJS-9 monoclonal antibody reacts with the mouse, human and rhesus monkey RORgamma(t) protein. RORgamma is a member of the retinoic acid-related orphan receptor (ROR) family, which also includes RORalpha and RORbeta. ROR family proteins are ligand-dependent transcription factors that play roles in multiple physiological processes. RORgamma is expressed in several tissues including liver, lung, muscle, heart and kidney. Furthermore, it was discovered that alternative transcription results in the expression of an isoform, RORgammat, which is expressed exclusively in cells of the lymphoid compartment, namely CD4+CD8+ double-positive thymoyctes, Th17 cells of the periphery and lymphoid tissue inducer (Lti) cells of lymphoid organs. The RORgammat isoform differs from RORgamma by three unique amino acids at its amino terminus. Therefore, the AFKJS-9 antibody will react with both the RORgamma and RORgammat isoforms.Applications Reported: This AFKJS-9 antibody has been reported for use in chromatin immunoprecipitation and western blotting.Applications Tested: This AFKJS-9 antibody has been tested by western blot analysis. This can be used at less than or equal to 5 μg per mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2μm post-manufacturing filtered.

Specifications

• Antigen: ROR gamma (t)
• Classification: Monoclonal
• Concentration: 0.5mg/mL
• Formulation: PBS with 0.09% sodium azide; pH 7.2
• Gene Accession No.: P51449, P51450
• Gene Alias: MGC129539, NR1F3, RORG, RZR-GAMMA, RZRG, TOR
• Host Species: Rat
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Type: Antibody
• Monoclonal or Polyclonal: Monoclonal
• Target Species: Murine

• Applications: Western Blot
• Clone: AFKJS-9
• Conjugate: Unconjugated
• Gene: RORC
• Format: Purified
• Gene Symbols: RORC
• Isotype: IgG2a
• Quantity: 25μg
• Storage Requirements: Store at 2-8°C.
• Primary or Secondary: Primary
• Gene ID (Entrez): 6097

For Research Use Only.