Thermo Scientific™ Pierce™ Rat TNF alpha ELISA Kits

Measure levels of rat tumor necrosis factor alpha (TNFa) levels in rat serum, plasma and culture supernatant samples using 96-well plates.

Overview
Brand: Thermo Scientific™ Pierce™

Manufacturer Part Number: ER3TNFA

UNSPSC: 41116126

Code: PN

Additional Details:
Additional Details: Weight: 0.10000kg



Product Code. 10741585

Quantity Price
1 £ 416.0 / 1 set
Estimated Shipment date
from Supplier 04-04-2017
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Description and Specification

Specification

Specificity No significant cross-reaction with human TNFα, human TNFβ or porcine TNFα. Mouse TNFα cross-reacts weakly (< 3%)
Precision Intra-assay CV <10%; inter-assay CV <10%
Sensitivity <15pg/mL
Formulation Complete ELISA kit with coated 96-well plate
Measurement Absorbance at 450nm minus absorbance at 550nm
Kit Contents 30X Wash Buffer, 50mL
Assay Range 31 to 2500pg/mL
Sufficient For 96 microplate wells
Format Colorimetric ELISA (TMB substrate) in clear 96-well strip plate
Target Natural and recombinant rat TNFa (rat tumor necrosis factor alpha)
Sample Type Cell culture supernatant, serum, plasma (EDTA, sodium citrate and heparin)
Sample Size 50µL
Description Rat TNF alpha ELISA Kit
Total Assay Time 3 hr.
Quantity 1 plate kit

These rat TNF alpha ELISA kits use pre-titered, matched pairs of coating and detection antibodies to achieve sensitive and accurate measurement of tumor necrosis factor alpha (TNF-alpha, TNFα) in samples at 50μL per well. Also included are calibrated recombinant protein standard, streptavidin-HRP, TMB substrate solution and stop solution. The complete kits contain precoated 96-well strip plate(s) and lot-optimized reagents. All components undergo quality control testing to minimize lot-to-lot variability.
  • Target: Natural and recombinant rat TNFα (rat tumor necrosis factor alpha)

  • Format: Colorimetric ELISA (TMB substrate) in clear 96-well strip plate

  • Measurement: Absorbance at 450nm minus absorbance at 550nm

  • Assay Range: 31 to 2500pg/mL

  • Sensitivity: < 15pg/mL

  • Precision: Intra-assay CV < 10%; inter-assay CV < 10%

  • Sample Types: Cell culture supernatant, serum, plasma (EDTA, sodium citrate and heparin)

  • Total Assay Time: 3 hours

  • Sample Size: 50μL

  • Specificity: No significant cross-reaction with human TNFα, human TNFβ or porcine TNFα. Mouse TNFα cross-reacts weakly (< 3%)

Includes:Complete kits contain pre-coated antibody-plates, detection antibodies, buffers, diluents, standards, and substrates.

Documentation

Technical documents