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Thermo Scientific™ Pierce™ Rat TNF alpha ELISA Kits

Measure levels of rat tumor necrosis factor alpha (TNFa) levels in rat serum, plasma and culture supernatant samples using 96-well plates.

Brand:  Thermo Scientific™ Pierce™ ER3TNFA

UNSPSC : 41116126

Code : PN

Additional Details : Weight : 0.10000kg

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Product Code. 10741585

  • £416.00 / 1 set


Estimated Shipment
06-07-2017
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Specifications

Description

These rat TNF alpha ELISA kits use pre-titered, matched pairs of coating and detection antibodies to achieve sensitive and accurate measurement of tumor necrosis factor alpha (TNF-alpha, TNFα) in samples at 50μL per well. Also included are calibrated recombinant protein standard, streptavidin-HRP, TMB substrate solution and stop solution. The complete kits contain precoated 96-well strip plate(s) and lot-optimized reagents. All components undergo quality control testing to minimize lot-to-lot variability.
  • Target: Natural and recombinant rat TNFα (rat tumor necrosis factor alpha)

  • Format: Colorimetric ELISA (TMB substrate) in clear 96-well strip plate

  • Measurement: Absorbance at 450nm minus absorbance at 550nm

  • Assay Range: 31 to 2500pg/mL

  • Sensitivity: < 15pg/mL

  • Precision: Intra-assay CV < 10%; inter-assay CV < 10%

  • Sample Types: Cell culture supernatant, serum, plasma (EDTA, sodium citrate and heparin)

  • Total Assay Time: 3 hours

  • Sample Size: 50μL

  • Specificity: No significant cross-reaction with human TNFα, human TNFβ or porcine TNFα. Mouse TNFα cross-reacts weakly (< 3%)

Includes:Complete kits contain pre-coated antibody-plates, detection antibodies, buffers, diluents, standards, and substrates.

Description & Specifications

Specifications

No significant cross-reaction with human TNFα, human TNFβ or porcine TNFα. Mouse TNFα cross-reacts weakly (< 3%)
<15pg/mL
Absorbance at 450nm minus absorbance at 550nm
31 to 2500pg/mL
Colorimetric ELISA (TMB substrate) in clear 96-well strip plate
Cell culture supernatant, serum, plasma (EDTA, sodium citrate and heparin)
Rat TNF alpha ELISA Kit
1 plate kit
Intra-assay CV <10%; inter-assay CV <10%
Complete ELISA kit with coated 96-well plate
30X Wash Buffer, 50mL
96 microplate wells
Natural and recombinant rat TNFa (rat tumor necrosis factor alpha)
50┬ÁL
3 hr.
Documentation

Technical Documents