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Invitrogen™ Rat TNF alpha ELISA Kits

Measure levels of rat tumor necrosis factor alpha (TNFa) levels in rat serum, plasma and culture supernatant samples using 96-well plates.

Brand:  Invitrogen™ ER3TNFA

Code : PN

Additional Details : Weight : 0.10000kg

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Product Code. 10741585

This item has been discontinued and is no longer available. View the product for possible alternatives or contact our Product Support team on 01509 555888 for assistance


Description

Description

These rat TNF alpha ELISA kits use pre-titered, matched pairs of coating and detection antibodies to achieve sensitive and accurate measurement of tumor necrosis factor alpha (TNF-alpha, TNFα) in samples at 50μL per well. Also included are calibrated recombinant protein standard, streptavidin-HRP, TMB substrate solution and stop solution. The complete kits contain precoated 96-well strip plate(s) and lot-optimized reagents. All components undergo quality control testing to minimize lot-to-lot variability.
  • Target: Natural and recombinant rat TNFα (rat tumor necrosis factor alpha)

  • Format: Colorimetric ELISA (TMB substrate) in clear 96-well strip plate

  • Measurement: Absorbance at 450nm minus absorbance at 550nm

  • Assay Range: 31 to 2500pg/mL

  • Sensitivity: < 15pg/mL

  • Precision: Intra-assay CV < 10%; inter-assay CV < 10%

  • Sample Types: Cell culture supernatant, serum, plasma (EDTA, sodium citrate and heparin)

  • Total Assay Time: 3 hours

  • Sample Size: 50μL

  • Specificity: No significant cross-reaction with human TNFα, human TNFβ or porcine TNFα. Mouse TNFα cross-reacts weakly (< 3%)

Includes:Complete kits contain pre-coated antibody-plates, detection antibodies, buffers, diluents, standards, and substrates.

Specifications

Specifications

31 to 2500pg/mL
Colorimetric ELISA (TMB substrate) in clear 96-well strip plate
30X Wash Buffer, 50mL, Anti-Rat TNF alpha Precoated 96-well Strip Plate, 1 ea, Biotinylated Antibody Reagent, 6mL, Lyophilized Recombinant Rat TNF alpha Standard, 2 vials, Pre-Treatment Buffer, 6mL, Standard Diluent, 25mL, Stop Solution, 13mL, Streptavidin-HRP Reagent, 14mL, TMB Substrate Solution, 13mL
Absorbance at 450nm minus absorbance at 550nm
<15pg/mL
50┬ÁL
96 microplate wells
Natural and recombinant rat TNFa (rat tumor necrosis factor alpha)
Rat TNF alpha ELISA Kit
Complete ELISA kit with coated 96-well plate
Intra-assay CV <10%; inter-assay CV <10%
1 plate kit
Cell culture supernatant, serum, plasma (EDTA, sodium citrate and heparin)
No significant cross-reaction with human TNFα, human TNFβ or porcine TNFα. Mouse TNFα cross-reacts weakly (< 3%)
3 hr.
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