Thermo Scientific™ Pierce™ Rat TNF alpha ELISA Kits

Measure levels of rat tumor necrosis factor alpha (TNFa) levels in rat serum, plasma and culture supernatant samples using 96-well plates.

Overview
Brand: Thermo Scientific™ Pierce™

Manufacturer Part Number: ER3TNFA5

RAT TNFA ELISA

UNSPSC: 41116126

Code: PN

Additional Details:
Additional Details: Weight: 0.10000kg



Product Code. 10290254

Quantity Price
1 £ 1354.0 / 1 set
Estimated Shipment date
from Supplier 03-01-2017
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Description and Specification

Specification

Assay Range 31 to 2500pg/mL
Description Rat TNF alpha ELISA Kit
Format Colorimetric ELISA (TMB substrate) in clear 96-well strip plate
Formulation Complete ELISA kit with coated 96-well plates
Kit Contents 30X Wash Buffer, 200mL
Precision Intra-assay CV <10%; inter-assay CV <10%
Measurement Absorbance at 450nm minus absorbance at 550nm
Quantity 5 plate kit
Sensitivity <15pg/mL
Sample Type Cell culture supernatant, serum, plasma (EDTA, sodium citrate and heparin)
Sample Size 50┬ÁL
Specificity No significant cross-reaction with human TNFα, human TNFβ or porcine TNFα. Mouse TNFα cross-reacts weakly (< 3%)
Sufficient For 5 x 96 microplate wells
Total Assay Time 3 hr.
Target Natural and recombinant rat TNFa (rat tumor necrosis factor alpha)

These rat TNF alpha ELISA kits use pre-titered, matched pairs of coating and detection antibodies to achieve sensitive and accurate measurement of tumor necrosis factor alpha (TNF-alpha, TNFα) in samples at 50μL per well. Also included are calibrated recombinant protein standard, streptavidin-HRP, TMB substrate solution and stop solution. The complete kits contain precoated 96-well strip plate(s) and lot-optimized reagents. All components undergo quality control testing to minimize lot-to-lot variability.
  • Target: Natural and recombinant rat TNFα (rat tumor necrosis factor alpha)

  • Format: Colorimetric ELISA (TMB substrate) in clear 96-well strip plate

  • Measurement: Absorbance at 450nm minus absorbance at 550nm

  • Assay Range: 31 to 2500pg/mL

  • Sensitivity: < 15pg/mL

  • Precision: Intra-assay CV < 10%; inter-assay CV < 10%

  • Sample Types: Cell culture supernatant, serum, plasma (EDTA, sodium citrate and heparin)

  • Total Assay Time: 3 hours

  • Sample Size: 50μL

  • Specificity: No significant cross-reaction with human TNFα, human TNFβ or porcine TNFα. Mouse TNFα cross-reacts weakly (< 3%)

Includes:Complete kits contain pre-coated antibody-plates, detection antibodies, buffers, diluents, standards, and substrates.

Documentation

Technical documents