Invitrogen™ TIMP-1 Rat ELISA Kit

Sandwich ELISA Kit

Brand:  Invitrogen™ ERTIMP1

Product Code. 15465393

Includes: Pre-coated plate(s), standard, detector antibody, HRP conjugate, diluents, wash buffer, chromogen, stop solution, and plate covers.

Description

The Rat Tissue Inhibitor of Matrix MetalloProteinase 1 (Rt TIMP-1) ELISA quantitates Rt TIMP-1 in rat serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Rt TIMP-1. Principle of the method The Rat TIMP-1 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Tissue inhibitor of metalloproteinases-1 (TIMP-1) and Tissue inhibitor of metalloproteinases-2 (TIMP-2) have similar properties, specifically in inhibiting enzymes of matrix metalloproteinase family, and are thought to be of great importance in the maintenance of connective tissue integrity. TIMP-1 forms a complex of 1:1 stoichiometry with activated interstitial collagenases, activated stromelysin, active form of 72kDa Type IV collagenase (also known as MMP-2 or gelatinase A), and latent and active forms 92kDa Type IV collagenase (also known as MMP-0 or gelatinase B). TIMPs inhibit the proteolytic invasiveness of tumor cells and normal placental trophoblast cells.

Specifications

• Accession Number: P30120
• Assay Sensitivity: 4 pg/mL
• Assay: Solid-phase sandwich ELISA
• Product Type: ELISA Kit
• Sample Type: Plasma, Serum, Supernatant
• Sufficient For: 96 assays
• For Use With (Application): ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
• Gene ID (Entrez): 116510
• Interassay CV: <12%
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Quantity: 96 Tests
• Research Discipline: Oncology
• Storage Requirements: 2°C to 8°C
• Target: Rat
• Total Assay Time: 4 hr. 45 min.

• Assay Range: 4.1-3000 pg/mL
• Calibration Range: 4pg/mL to 3000pg/mL
• Conjugate: Biotin
• Protein Family: Cytokines & Receptors
• Specificity: This ELISA kit shows no cross-reactivity with any of the cytokines tested: rat CINC-2, CINC-3, CNTF, Fractalkine, IL-1 alpha, IL-1 beta, IL-4, IL-6, IL-10, GM-CSF, IFN-gamma, LIX, Leptin, MCP-1, MIP-3 alpha, beta- NGF, TNF-alpha, VEGF.
• Target Species: Rat
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene Symbol: Timp1
• Intraassay CV: <10%
• Label or Dye: HRP
• Regulatory Status: RUO
• Sample Volume: Plasma, 3 μL; Serum, 3 μL; Supernatant, 100 μL
• Synonym: TIMP-1, Timp
• Test Time: 1 hr. 20 min.