Learn More
Invitrogen™ Rat MCP-1 ELISA Kits
Measure levels of rat monocyte chemoattractant protein-1 (MCP1) in rat serum, plasma and cell culture supernatant samples.
Brand: Invitrogen™ ERMCP1
Code : PN
Additional Details : Weight : 0.10000kg
Description
Assay MCP-1 (rat monocyte chemoattractant protein-1) levels in rat serum, plasma (EDTA, heparin, sodium citrate) and culture supernatant samples with our Thermo Scientific Rat MCP-1 ELISA Kits.
These rat MCP-1 ELISA kits use pre-titered, matched pairs of coating and detection antibodies to achieve sensitive and accurate measurement of monocyte chemoattractant protein-1 (MCP-1) in samples at 50μL per well. Also included are calibrated recombinant protein standard, streptavidin-HRP, TMB substrate solution and stop solution. The complete kits contain pre-coated 96-well strip plate(s) and lot-optimized reagents. All components undergo quality control testing to minimize lot-to-lot variability.Target: Natural and recombinant rat MCP-1 (monocyte chemoattractant protein-1)
Format: Colorimetric ELISA (TMB substrate) in clear 96-well strip plate
Measurement: Absorbance at 450nm minus absorbance at 550nm
Assay Range: 38 to 1500pg/mL
Sensitivity: < 5pg/mL
Precision: Intra-assay CV < 10%; inter-assay CV < 10%
Sample Types: Cell culture supernatant, serum, plasma (EDTA, sodium citrate and heparin)
Total Assay Time: 3 hours
Sample Size: 50μL
Specificity: No significant cross-reaction with rat IFNγ, IL-1α, IL-1β, IL-2, IL-4, IL-6, IL-10, MIP-1α, RANTES, mouse MCP-1, or human MCP-1
Includes:Complete kits contain pre-coated antibody-plates, detection antibodies, buffers, diluents, standards, and substrates.
Specifications
38 to 1500pg/mL | |
Rat MCP-1 ELISA Kit | |
Cell Culture Supernatant, Plasma (EDTA, Citrate, Heparin), Serum | |
96 microplate wells | |
Absorbance at 450nm minus absorbance at 550nm | |
1 Plate Kit | |
Natural and recombinant rat MCP-1 (monocyte chemoattractant protein-1) |
<5pg/mL | |
Colorimetric ELISA (TMB substrate) with Plates | |
No significant cross-reaction with rat IFNg, IL-1a, IL-1ß, IL-2, IL-4, IL-6, IL-10, MIP-1a, RANTES, mouse MCP-1, or human MCP-1 | |
Lyophilized Recombinant Rat MCP-1 Standard, 2 vials, TMB Substrate Solution, 13mL, Streptavidin-HRP Concentrate, 75µL, Standard Diluent, 25mL, HRP Dilution Buffer, 14mL, Biotinylated Antibody Reagent, 8mL, Anti-Rat MCP-1 Precoated 96-well Strip Plate, 1 ea, Adhesive Plate Covers, 6 ea, 30X Wash Buffer, 50mL, Stop Solution, 13mL | |
Intra-assay CV <10%; inter-assay CV <10% | |
50 μL | |
3 hr. |