Invitrogen™ IL-6 Rat ELISA Kit

For the quantitative detection of rat IL-6.

Brand:  Invitrogen™ BMS625

Product Code. 13467093

Includes: Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to rat IL-6
Biotin-Conjugate anti-rat IL-6 monoclonal antibody
Streptavidin-HRP
Rat IL-6 Standard lyophilized, 4ng/mL upon reconstitution
Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
Substrate Solution (tetramethyl-benzidine)
Stop Solution (1M Phosphoric acid)
Blue-Dye
Green-Dye
Red-Dye
Adhesive Films

Description

Interleukin 6 (IL-6) is a multifunctional 26 kD protein originally discovered in the medium of RNA-stimulated fibroblastoid cells. IL-6 appears to be directly involved in the responses that occur after infection and cellular injury, and it may prove to be important as IL-1 and TNF-a in regulating the acute phase response. IL-6 is reported to be produced by fibroblasts, activated T cells, activated monocytes or macrophages and endothelial cells. IL-6 acts upon a variety of cells including myeloid progenitor cells, T cells, B cells, and hepatocytes. In addition, IL-6 appears to interact with IL-2 in the proliferation of T lymphocytes. IL-6 potentiates the proliferative effect of IL-3 on multipotential hematopoietic progenitors. IL-6 plays a critical role in B-cell differentiation to plasma cells and is a potent growth factor for plasmacytoma and myeloma. IL-6 is a very useful culture supplement for the generation of a high number of antibody-producing hybridomas. Primarily produced at sites of acute and chronic inflammation, IL-6 is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of IL-6 is implicated in a wide variety of inflammation-associated disease states including diabetes mellitus and systemic juvenile rheumatoid arthritis.

Specifications

• Accession Number: P20607
• Conjugate: Biotin
• Gene ID (Entrez): 24498
• Kit Contents: Pre-coated 96 well plate, Standard, Sample Diluent, Assay Buffer concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Regulatory Status: RUO
• Sample Size: 50 μL
• Storage Requirements: 2°C to 8°C
• Total Assay Time: 3 hr. 10 min.
• Gene Symbols: ILg6,Ifnb2
• Interassay CV: 10%
• Label or Dye: HRP
• Target Species: Rat

• Assay Range: 31.3-2,000 pg/mL
• For Use With (Application): ELISA
• For Use With (Equipment): Colorimetric Microplate Reader
• Product Type: ELISA Kit
• Sensitivity: 12.0 pg/mL
• Sample Volume: Serum, 50 μL|Supernatant, 50 μL
• Test Time: 1 hr. 20 min.
• Target: Rat
• Synonym: Interferon-beta 2, B-cell stimulatory factor 2,HGF
• Intraassay CV: 5%
• Quantity: 96 Tests
• Sample Type: Supernatant

For Research Use Only