Thermo Scientific™ Pierce™ Rat IL-6 ELISA Kits

Measure levels of rat interleukin-6 in rat serum, plasma (EDTA, heparin, sodium citrate) and culture supernatant samples using 96-well plates.

Overview
Brand: Thermo Scientific™ Pierce™

Manufacturer Part Number: ER3IL62

RAT IL-6 ELISA, 2 PLATE KIT

UNSPSC: 41116126

Code: PN

Additional Details:
Additional Details: Weight: 0.10000kg



Product Code. 10342060

Quantity Price
1 £ 443.0 / 1 set
Estimated Shipment date
from Supplier 03-01-2017
Add to basket


Description and Specification

Specification

Assay Range 62.5 to 4000pg/mL
Description Rat IL-6 ELISA Kit
Format Colorimetric ELISA (TMB substrate) in clear 96-well strip plate
Formulation Complete ELISA kit with coated 96-well plates
Kit Contents 20X Wash Buffer, 2 x 55mL
Precision Dependent upon sample type
Measurement Absorbance at 450nm minus absorbance at 550nm
Quantity 2 plate kit
Sensitivity <16pg/mL
Sample Type Cell culture supernatant, serum, plasma (EDTA, sodium citrate and heparin)
Sample Size 50µL
Specificity No significant cross-reaction with rat IL-10 and TNFa; mouse IL-6, IL-1ß, TNFa, IL-4, IL-2 and IL-1a; or human IL-8 and GM-CSF
Sufficient For 2 x 96 microplate wells
Total Assay Time 4 hr.
Target Natural and recombinant rat interleukin-6 (IL-6)

These rat IL-6 ELISA kits use pre-titered, matched pairs of coating and detection antibodies to achieve sensitive and accurate measurement of interleukin-6 (IL-6) in samples at 50μL per well. Also included are calibrated recombinant protein standard, streptavidin-HRP, TMB substrate solution and stop solution. The complete kits contain pre-coated 96-well strip plate(s) and lot-optimized reagents. All components undergo quality control testing to minimize lot-to-lot variability.
  • Target: Natural and recombinant rat interleukin-6 (IL-6)

  • Format: Colorimetric ELISA (TMB substrate) in clear 96-well strip plate

  • Measurement: Absorbance at 450nm minus absorbance at 550nm

  • Assay Range: 62.5 to 4000pg/mL

  • Sensitivity: < 16pg/mL

  • Precision: Dependent upon sample type

  • Sample Types: Cell culture supernatant, serum, plasma (EDTA, sodium citrate and heparin)

  • Total Assay Time: 4 hours

  • Sample Size: 50μL

  • Specificity: No significant cross-reaction with rat IL-10 and TNFα; mouse IL-6, IL-1β, TNFα, IL-4, IL-2 and IL-1α; or human IL-8 and GM-CSF

Includes:Complete kits contain pre-coated antibody-plates, detection antibodies, buffers, diluents, standards, and substrates.