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Invitrogen™ Rat IL-10 ELISA Kits

Measure levels of rat interleukin-10 in rat serum, plasma (EDTA, heparin, sodium citrate) and cell culture supernatant samples using 96-well plates.

Brand:  Invitrogen™ ERIL10

Code : PN

Additional Details : Weight : 0.45000kg Transport : UN number : 3265 Chem class : 1a Pack group : III

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Product Code. 10677334

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Description

Description

These rat IL-10 ELISA kits use pre-titered, matched pairs of coating and detection antibodies to achieve sensitive and accurate measurement of interleukin-10 (IL-10) in samples at 50μL per well. Also included are calibrated recombinant protein standard, streptavidin-HRP, TMB substrate solution and stop solution.The complete kits contain pre-coated 96-well strip plate(s) and lot-optimized reagents. All components undergo quality control testing to minimize lot-to-lot variability.
  • Target: Natural and recombinant rat interleukin-10 (IL-10)

  • Format: Colorimetric ELISA (TMB substrate) in clear 96-well strip plate

  • Measurement: Absorbance at 450nm minus absorbance at 550nm

  • Assay Range: 16 to 500pg/mL

  • Sensitivity: < 3pg/mL

  • Precision: Dependent upon sample type (generally less than 10% CV)

  • Sample Types: Cell culture supernatant, serum, plasma (EDTA, sodium citrate and heparin)

  • Total Assay Time: 3 hours

  • Sample Size: 50μL

  • Specificity: No significant cross-reaction with rat IFNγ, IL-1α, IL-1β, IL-2, IL-4, IL-6, MCP-1, MIP-1α, RANTES, TNFα, human IL-10, or mouse IL-10

Includes:Complete kits contain pre-coated antibody-plates, detection antibodies, buffers, diluents, standards, and substrates.

Specifications

Specifications

16 to 500pg/mL
Colorimetric ELISA (TMB substrate) in clear 96-well strip plate
30X Wash Buffer, 50mL
Absorbance at 450nm minus absorbance at 550nm
<3pg/mL
50µL
96 microplate wells
Natural and recombinant rat interleukin-10 (IL-10)
Rat IL-10 ELISA Kit
Complete ELISA kit with coated 96-well plate
Dependent upon sample type (generally less than 10% CV)
1 plate kit
Cell culture supernatant, serum, plasma (EDTA, sodium citrate and heparin)
No significant cross-reaction with rat IFNg, IL-1a, IL-1ß, IL-2, IL-4, IL-6, MCP-1, MIP-1a, RANTES, TNFa, human IL-10, or mouse IL-10
3 hr.
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