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Invitrogen™ IL-1 beta Rat ELISA Kit

For the quantitative detection of rat IL-1beta.

Brand:  Invitrogen™ BMS630TEN

Code : NEW

Additional Details : Weight : 0.23750kg

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Product Code. 15572067

  • £1672.00 / Pack of 10


Estimated Shipment date
from Supplier 05-03-2020
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Includes: Aluminium pouch(es) with a Microwell Plate coated with polyclonal antibody to rat IL-1beta
Biotin-Conjugate anti-rat IL-1beta polyclonal antibody
Streptavidin-HRP
Rat IL-1beta Standard lyophilized, 4ng/mL upon reconstitution
Sample Diluent
Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
Substrate Solution (tetramethyl-benzidine)
Stop Solution (1M Phosphoric acid)
Blue-Dye
Green-Dye
Red-Dye
Adhesive Films

Description

Description

Interleukin-1 beta (IL-1 beta) is a proinflammatory cytokine expressed by monocytes, macrophages, and dendritic cells. IL-1 beta is synthesized in response to inflammatory stimuli as a 31 kDa inactive pro-form that accumulates in the cytosol. Cleavage of pro-IL-1 beta into the active 17 kDa protein requires the activation of inflammasomes, which are multi-protein complexes that respond to pathogens, stress conditions, and other danger signals. Inflammasome activation triggers the processing of the caspase-1 precursor into its active form, which in turn cleaves pro-IL-1 beta. IL-1 beta lacks a signal sequence peptide for classical ER/Golgi pathway and is secreted alongside caspase-1 via an alternate and incompletely understood mechanism. Although IL-1 beta is most often secreted in its active form, secretion of the uncleaved protein may be detectable under some biological conditions. IL-1 beta signals through two receptors, IL-1RI and IL-1RII, both of which are shared with IL-1 alpha. IL-1 beta activity can be moderated by IL-1 Receptor Antagonist (IL-1RA), a protein produced by many cell types that blocks receptor binding through competitive inhibition. IL-1 beta play an important role in innate host defense by triggering the production of other proinflammatory cytokines in target cells and initiating acute-phase responses to infection and injury. Elevated levels of IL-1 beta have been associated with many chronic inflammatory conditions IL-1 beta neutralizing antibodies potential therapeutic value.
Specifications

Specifications

Q63264
Biotin
24494
Pre-coated 96 well plate, Standard, Sample Diluent, Assay Buffer concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
RUO
50 μL
2°C to 8°C
3 hr. 10 min.
Il1b
10%
HRP
Rat
31.3-2,000 pg/mL
ELISA
Colorimetric Microplate Reader
ELISA Kit
4.0 pg/mL
Serum, 50 μL|Supernatant, 50 μL
1 hr. 20 min.
Rat
Catabolin, Interleukin-1 beta, IL1F2
10%
10 x 96 Tests
Supernatant
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For Research Use Only