Invitrogen™ CRP Rat ELISA Kit

Sandwich ELISA Kit

Brand:  Invitrogen™ ERCRP

Product Code. 15485363

Description

The Rat C-Reactive Protein (Rt CRP) ELISA quantitates Rt CRP in rat serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Rt CRP. Principle of the method The Rat CRP solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

CRP (C-reactive protein) is a major cyclic, pentameric acute phase protein compound consisting of five identical, noncovalently bound, nonglycosylated subunits. Eeach subunit is made up of 206 amino acids, and has a molecular weight of 24 kDa. CRP is produced by the liver, and its plasma levels rise dramatically during inflammatory processes occurring in the body. CRP is an initiator of classical complement cascade, binds to several nuclear components (chromatin, histones, etc.), plays an important role in innate immunity, and mediates activities associated with pre-immune nonspecific host resistance. Specifically, there is a 100-1000 fold increase of CRP in response to injury, infection or inflammation. CRP is opsonic, an initiator of the classical complement cascade, and an activator of monocytes/macrophages. CRP also binds to several nuclear components including chromatin, histones and snRNP, suggesting that it may play a role as a scavenger during cell necrosis. CRP is involved in several host defense related functions based on its ability to recognize foreign pathogens and damaged cells of the host, and initiates pathogen elimination by interacting with humoral and cellular effector systems in the blood. Patients with elevated basal levels of CRP have been shown to be an increased risk for hypertension and cardiovascular disease.

Specifications

• Accession Number: P48199
• Assay Sensitivity: 200 pg/mL
• Description: CRP (C-reactive) protein ELISA Kit, 96 Assays, 1 Kit, Rat Species, Proteases, Peptidases and Inhibitors Protein Family, Immunology Research Discipline, Enzyme-Linked Immunosorbent Assay Designed for the Quantitation and Detection of C-Reactive Protein (CR
• Assay: Solid-phase sandwich ELISA
• Detection Method: Colorimetric
• Protein Family: Proteases, Peptidases & Inhibitors
• Specificity: This ELISA kit shows no cross-reactivity with the following cytokines tested: rat CINC-2, CINC-3, CNTF, Fractalkine, IL-1 alpha, IL-1 beta, IL-4, IL-6, IL-10, GM-CSF, IFN-gamma, Leptin, Lix, MCP-1, MIP-3 alpha, beta- NGF, TIMP-1, TNF-alpha.
• Sufficient For: 96 Assays
• For Use With (Application): ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
• Gene ID (Entrez): 25419
• Host Species: Rat
• Intraassay CV: <10%
• Label or Dye: HRP
• Regulatory Status: RUO
• Sample Volume: Plasma, 1 μL; Serum, 1 μL; Supernatant, 100 μL
• Synonym: Aa1249, Ab1-341, Ab2-196, Ac1-114, Ac1262, Ac2-069, Ba2-693
• Test Time: 1 hr. 20 min.

• Assay Range: 0.246-60 ng/mL
• Calibration Range: 0.2ng/mL to 60ng/mL
• Detectable Analytes: C-reactive protein (CRP)
• Conjugate: Biotin
• Product Type: ELISA Kit
• Sample Type: Plasma, Serum, Supernatant
• Substrate Type: TMB
• Target Species: Rat
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene Symbol: PTX1
• Interassay CV: <12%
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers, Anti-Rat C-reactive protein precoated 96-well strip plate, lyophilized recombinant rat C-reactive protein standard, 20X wash buffer, biotinylated antibody reagent, streptavidin-HRP reagent, TMB substrate, stop solution, contains 0.2M sulfuric acid, assay
• Quantity: 96 Tests
• Research Discipline: Immunology
• Storage Requirements: 2°C to 8°C
• Target: Rat
• Total Assay Time: 4 hr. 45 min.