RASSF1A, clone: eB114-10H1, eBioscience™
Mouse Monoclonal Antibody
Brand: Affymetrix eBioscience 14-6888-80
Code : Z2
Additional Details : Weight : 0.01000kg
DescriptionDescription: The eB114 antibody reacts with the RASSF1A isoform of the tumor suppressor gene RASSF1. RASSF1A contains a Ras Association Domain (RA) and binds Ras in a GTP-dependent manner to effect apoptosis. The RASSF1A isoform undergoes epigenetic inactivation in a variety of human primary tumors. This has been associated with hypermethylation of a CpG island(s) in its promoter region. This antibody was generated against a synthetic peptide corresponding to the splice junction region of RASSF1A.Applications Reported: The eB114 antibody has been reported for use in immunoprecipitation, and immunoblotting (WB).Applications Tested: The eB114-10H1 has been tested by Western blotting and can be used at approximately 1-10 μg/mL. RASS1A is detected as a 45kDa band in Western blotting of a HeLa cell extract. It is recommended that the reagent be carefully titrated for optimal performance in the assay of interest.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2μm post-manufacturing filtered.
|aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer|
|Store at 2-8°C.|