R&D Systems™ Mouse Osteopontin/OPN Recombinant Protein
Brand: R&D Systems™ 441-OP-050/CF
Code : Z1
Additional Details : Weight : 0.00010kg
DescriptionMeasured by the ability of the immobilized protein to support the adhesion of HEK293 human embryonic kidney cells. Agnihotri, R. et al. (2001) J. Biol. Chem. 276:28261. When 1 x 10 5 cells/well are added to a Recombinant Mouse (rm) Osteopontin/OPN coated plate, cell adhesion is enhanced in a dose-dependent manner after 1 hour incubation at 37 degreesC. The ED 50 for this effect is 0.25-1.5 µg/mL. Recombinant Human Coagulation Factor II/Thrombin (Catalog # 1473-SE ) proteolytic treatment of this rmOsteopontin can increase HEK293 cell adhesion by about 10-fold.
|Lyophilized from a 0.2μm filtered solution in PBS.|
|95%, by SDS-PAGE under reducing conditions and visualized by silver stain.|
|Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70° C as supplied. 3 months, -20 to -70° C under sterile conditions after reconstitution.|