R&D Systems™ Human Wnt-3a Recombinant Protein
Brand: R&D Systems™ 5036-WN-010
Code : Z1
Additional Details : Weight : 0.00010kg
DescriptionMeasured by its ability to induce alkaline phosphatase production by MC3T3-E1 mouse preosteoblast cells. The ED 50 for this effect is 5-25 ng/mL. Measured by its ability to induce Topflash reporter activity in HEK293T human embryonic kidney cells. The ED 50 for this effect is <500 ng/mL. Protein concentrations should be titrated based on cell type and if appropriate, passage number of the cell line. Optimal concentrations should be determined by each laboratory for each application.
|Lyophilized from a 0.2μm filtered solution in PBS, EDTA and CHAPS with BSA as a carrier protein.|
|75%, by SDS-PAGE with silver staining.|
|Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70° C as supplied. 1 month, 2 to 8° C under sterile conditions after reconstitution. 3 months, -20 to -70° C under sterile conditions after reconstitution.|