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Applied Biosystems™ PowerUp™ SYBR™ Green Master Mix

Pre-formulated, optimized, universal 2X master mix for real-time PCR workflows.

Brand:  Applied Biosystems™ A25779

Code : NEW

Additional Details : Weight : 0.00200kg

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Product Code. 15366158

  • £105.00 / Pack of 2


Estimated Shipment date
from Supplier 03-01-2018
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Disclaimers:

For Research Use Only. Not for use in diagnostic procedures.


Description

Description

  • A dual hot-start mechanism for excellent specificity
  • Highly reproducible CTs over a broad dynamic range
  • Inclusion of UDG to help prevent carryover contamination
  • Stability of pre-assembled reactions for up to 72 hours
  • Compatibility with most real-time qPCR instruments

Exceptional specificity with dual hot-start mechanism:
  • Specificity is paramount in obtaining high-quality data in SYBR™ Green reactions and can be enhanced through control of the Taq DNA polymerase at lower temperatures before stringent primer binding
  • PowerUp™ SYBR™ Green Master Mix uses the Dual-Lock™ Taq DNA polymerase enzyme that combines two unique hot-start mechanisms to help control its activity and to prevent undesirable early activity of the polymerase at low temperatures
  • In an experiment evaluating 24 different primer sets, PowerUp™ SYBR™ Green Master Mix generated single melt curves 100% of the time, consistent with highly specific amplification

Tight reproducibility in CTs over a wide dynamic range:
  • PowerUp™ SYBR™ Green Master Mix demonstrates excellent reproducibility over a wide dynamic range for a variety of targets tested
  • In addition, this master mix provides efficient amplification over 6 logs of sample input
  • Unlike competitor mixes that can exhibit inhibition at high cDNA inputs and low correlation coefficients over a dynamic range experiment, PowerUp™ SYBR™ Green Master Mix efficiency is maintained over the full dynamic range to help deliver maximal confidence in data quality

UDG included for carryover contamination control:
  • The inclusion of uracil-DNA glycosylase (UDG) and dUTP in the PowerUp™ SYBR™ Green Master Mix enables the degradation of any previously amplified PCR products, helping prevent contamination of subsequent qPCR reactions and possible false positives

Stability of pre-assembled reactions:
  • The tight control of the Dual-Lock™ Taq DNA polymerase in PowerUp™ SYBR™ Green Master Mix enables reactions to be assembled up to 72 hours prior to cycling without impacting data
  • This consistency allows users to have confidence and flexibility in the use of PowerUp™ SYBR™ Green Master Mix across numerous workflow scenarios1

Broad instrument compatibility:
  • PowerUp™ SYBR™ Green Master Mix can be used in either standard or fast cycling mode and is compatible with all Applied Biosystems™ real-time PCR instruments2
  • It is also compatible with the Bio-Rad IQ™5 and CFX96™/CFX384™ systems, Roche LightCycler™ LC480, and Stratagene™ MX3005P™ systems
  • For optimal results, recommended primer concentrations are 300 to 800nM

Order Info

Shipping Conditions: Wet Ice

Specifications

Specifications

PowerUp™ SYBR™ Green Master Mix
The 2X mix contains SYBR Green dye, Dual-Lock Taq DNA Polymerase, dNTPs with dUTP/dTTP blend, heat-labile UDG, ROX passive reference dye, and optimized buffer components. Contains 2 x 1mL tube, sufficient for 200 20μL reactions (10μL Master Mix per reaction).
2 x 1mL
PowerUp™ SYBR™ Green Master Mix, 2 x 1mL, 200 rxns, DNA General Sample Type, SYBR Detection Method, Fast or Standard Reaction Speed, 2 to 8°C Storage Temperature, qPCR PCR Method, Dual-Lock™ Taq DNA Polymerase
7300 System, 7500 Fast Dx System, 7500 Fast System, 7700 System, 7900HT Fast System, Agilent Mx3005P system, Bio-Rad CFX Connect™, Bio-Rad CFX384 Touch™, Bio-Rad CFX96 Touch™, QuantStudio™ 12K Flex, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6, QuantStudio™ 7, Roche LightCycler 480, Roche LightCycler 96, StepOne™ System, StepOnePlus™ System, ViiA™ 7 System
Fast or Standard
Real time PCR (qPCR)
200 Reactions
Store at 2° to 8°C.
Detection Method: SYBR; PCR Method: qPCR
DNA
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Special Offers

Special Offers

  • This issue of Life in the Lab, “Illuminating the immune system”
    : 12863

    2018-01-31

    *Discount will apply to qualifying orders received by Fisher Scientific between 19/10/2017 and 31/01/2018. Promotion code must be presented at the time of order. Discount applies to the list price in effect at the time the order is received. Promotion cannot be combined with other discounts or promotions. Offer void where prohibited, licensed, or restricted by federal, state, provincial, or local laws or regulation or agency/institutional policy. Other restrictions may apply.

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