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Invitrogen™ Porcine TNF alpha ELISA Kits, Thermo Scientific™

Measure levels of porcine tumor necrosis factor alpha (TNFa) in pig serum, plasma and cell culture supernatant samples using 96-well plates.

Brand:  Invitrogen™ EP2TNFA

Code : PN

Additional Details : Weight : 0.10000kg

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Product Code. 10210594

  • £409.00 / 1 set


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Description

Description

These porcine TNF alpha ELISA kits use pre-titered, matched pairs of coating and detection antibodies to achieve sensitive and accurate measurement of tumor necrosis factor alpha (TNF-alpha, TNFα) in samples at 50 to 100μL per well. Also included are calibrated recombinant protein standard, streptavidin-HRP, TMB substrate solution and stop solution. The complete kits contain precoated 96-well strip plate(s) and lot-optimized reagents. The economically-priced reagent kit provides validated reagents only, requiring users to coat their own plates. All components undergo quality control testing to minimize lot-to-lot variability.
  • Target: Natural and recombinant porcine TNFα (porcine tumor necrosis factor alpha)

  • Format: Colorimetric ELISA (TMB substrate) in clear 96-well strip plate

  • Measurement: Absorbance at 450nm minus absorbance at 550nm

  • Assay Range: 31.3 to 2000pg/mL

  • Sensitivity: < 5pg/mL

  • Precision: Dependent upon sample type and level (see instructions)

  • Sample Types: Cell culture supernatant, serum, plasma (EDTA, sodium citrate and heparin)

  • Total Assay Time: 4 hours

  • Sample Size: 50μL

  • Specificity: No significant cross-reaction with human IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-11, IL-12p70, IL-12p40, IL-13, IL-15, IL-16, IL-17, IL-18, Eotaxin, GCSF, GMCSF, GROα, GROβ, IFNγ, IFNα, MCP-1, MCP-2, MCP-3, MCP-4, VEGF, MIP-1α, MIP-1β, TGFβ, TNFβ or RANTES; mouse TNFα; rat TNFα; bovine TNFβ. This ELISA uses antibodies raised against human TNFα. This ELISA reacts with porcine and human TNFα at 100%

Assay range and other values listed above are for the pre-coated plate kit; see product documentation for values specific to the reagent kit.

Includes:Complete Kits contain pre-coated antibody-plates, detection antibodies, buffers, diluents, standards, and substrates. The Reagent Kit (previously called a “Screening Set”) contains matched antibodies, standard and detection reagents but no coated plates.

Specifications

Specifications

31.3 to 2000pg/mL
Colorimetric ELISA (TMB substrate) in clear 96-well strip plate
30X Wash Buffer, 50mL
Absorbance at 450nm minus absorbance at 550nm
<5pg/mL
50µL
96 microplate wells
Natural and recombinant porcine TNFa (porcine tumor necrosis factor alpha)
Porcine TNF alpha ELISA Kit
Complete ELISA kit with coated 96-well plate
Dependent upon sample type and level (see instructions)
1 plate kit
Cell culture supernatant, serum, plasma (EDTA, sodium citrate and heparin)
No significant cross-reaction with human IL-1a, IL-1ß, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-11, IL-12p70, IL-12p40, IL-13, IL-15, IL-16, IL-17, IL-18, Eotaxin, GCSF, GMCSF, GROa, GROß, IFNg, IFNa, MCP-1, MCP-2, MCP-3, MCP-4, VEGF, MIP-1a, MI
4 hr.
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