missing translation for 'onlineSavingsMsg'
Learn More

Porcine TNF alpha ELISA Kits, Thermo Scientific™

Measure levels of porcine tumor necrosis factor alpha (TNFa) in pig serum, plasma and cell culture supernatant samples using 96-well plates.

Brand:  Invitrogen EP2TNFA

Code : PN

Additional Details : Weight : 0.10000kg

 View more versions of this product

Product Code. 10210594

This item has been discontinued and is no longer available. View the product for possible alternatives or contact our Product Support team on 01509 555888 for assistance
View alternative products »


Description

Description

These porcine TNF alpha ELISA kits use pre-titered, matched pairs of coating and detection antibodies to achieve sensitive and accurate measurement of tumor necrosis factor alpha (TNF-alpha, TNFα) in samples at 50 to 100μL per well. Also included are calibrated recombinant protein standard, streptavidin-HRP, TMB substrate solution and stop solution. The complete kits contain precoated 96-well strip plate(s) and lot-optimized reagents. The economically-priced reagent kit provides validated reagents only, requiring users to coat their own plates. All components undergo quality control testing to minimize lot-to-lot variability.
  • Target: Natural and recombinant porcine TNFα (porcine tumor necrosis factor alpha)

  • Format: Colorimetric ELISA (TMB substrate) in clear 96-well strip plate

  • Measurement: Absorbance at 450nm minus absorbance at 550nm

  • Assay Range: 31.3 to 2000pg/mL

  • Sensitivity: < 5pg/mL

  • Precision: Dependent upon sample type and level (see instructions)

  • Sample Types: Cell culture supernatant, serum, plasma (EDTA, sodium citrate and heparin)

  • Total Assay Time: 4 hours

  • Sample Size: 50μL

  • Specificity: No significant cross-reaction with human IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-11, IL-12p70, IL-12p40, IL-13, IL-15, IL-16, IL-17, IL-18, Eotaxin, GCSF, GMCSF, GROα, GROβ, IFNγ, IFNα, MCP-1, MCP-2, MCP-3, MCP-4, VEGF, MIP-1α, MIP-1β, TGFβ, TNFβ or RANTES; mouse TNFα; rat TNFα; bovine TNFβ. This ELISA uses antibodies raised against human TNFα. This ELISA reacts with porcine and human TNFα at 100%

Assay range and other values listed above are for the pre-coated plate kit; see product documentation for values specific to the reagent kit.

Includes:Complete Kits contain pre-coated antibody-plates, detection antibodies, buffers, diluents, standards, and substrates. The Reagent Kit (previously called a “Screening Set”) contains matched antibodies, standard and detection reagents but no coated plates.

Specifications

Specifications

31.3 to 2000pg/mL
Porcine TNF alpha ELISA Kit
Complete ELISA kit with coated 96-well plate
Absorbance at 450nm minus absorbance at 550nm
<5pg/mL
50µL
96 microplate wells
Natural and recombinant porcine TNFa (porcine tumor necrosis factor alpha)
30X Wash Buffer, 50mL
Colorimetric ELISA (TMB substrate) in clear 96-well strip plate
Dependent upon sample type and level (see instructions)
1 plate kit
Cell culture supernatant, serum, plasma (EDTA, sodium citrate and heparin)
No significant cross-reaction with human IL-1a, IL-1ß, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-11, IL-12p70, IL-12p40, IL-13, IL-15, IL-16, IL-17, IL-18, Eotaxin, GCSF, GMCSF, GROa, GROß, IFNg, IFNa, MCP-1, MCP-2, MCP-3, MCP-4, VEGF, MIP-1a, MI
4 hr.
Documentation

Technical Documents

Alternative Products