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Invitrogen™ IL-17 (IL-17A) Porcine ELISA Kit
Sandwich ELISA Kit
Brand: Invitrogen™ ESIL17A
Code : NEW
Additional Details : Weight : 0.00400kg
Includes: Pre-coated plate(s), standard, detector antibody, HRP conjugate, diluents, wash buffer, chromogen, stop solution, and plate covers.
Description
The Porcine Interleukin 17 (IL-17A) ELISA quantitates Porc IL-17A in porcine serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Porc IL-17A. Principle of the method The Porcine IL-17A solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
Interleukin-17A (IL-17A, CTLA-8) is a CD4+ T cell-derived cytokine that promotes inflammatory responses in cell lines and is elevated in rheumatoid arthritis, asthma, multiple sclerosis, psoriasis, and transplant rejection. IL-17A is a 32 kDa long, disulfide-linked homodimer consisting of 136 amino acids that is a member of a six-species family of proteins (IL-17A-17F) and signals through the IL-17 receptor (IL-17R/CDw217). High levels of IL-17A homodimer are produced by activated peripheral blood CD4+ T-cells, and IL-17A also enhances expression of the intracellular adhesion molecule-1 (ICAM-1) in human fibroblasts. In particular, human IL-17A also stimulates epithelial, endothelial, or fibroblastic cells to secrete IL-6, IL-8, G-CSF, and PGE2. In the presence of human IL-17A, fibroblasts can sustain the proliferation of CD34+ hematopoietic progenitors and induce maturation into neutrophils. Mouse, rat, and human IL-17A can induce IL-6 secretion in mouse stromal cells, indicating that all homologs can recognize the mouse receptor. IL-17A regulates the activities of NF-kappa B and mitogen-activated protein kinases, stimulates the expression of IL-6 and cyclooxygenase-2 (PTGS2/COX-2), and enhances the production of nitric oxide (NO).Specifications
Q60I29 | |
14 pg/mL | |
Solid-phase sandwich ELISA | |
ELISA Kit | |
Plasma, Serum, Supernatant | |
96 assays | |
ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis | |
449530 | |
<12% | |
Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers | |
96 Tests | |
Inflammation | |
2°C to 8°C | |
Porcine | |
4 hr. 45 min. |
16.38-4000 pg/mL | |
14pg/mL to 4000pg/mL | |
Biotin | |
Cytokines & Receptors | |
This ELISA pair antibody also can recognize bovine and equine IL-17A. | |
Porcine | |
Colorimetric Microplate Reader | |
IL17 | |
<10% | |
HRP | |
RUO | |
Plasma, 50 μL; Serum, 50 μL; Supernatant, 100 μL | |
IL17 | |
1 hr. 20 min. |