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Invitrogen™ PolarScreen™ Glucocorticoid Receptor Competitor Assay Kit, Green

PolarScreen™ Glucocorticoid Receptor Competitor Assay Kit, Green

Brand:  Invitrogen™ A15897

Code : NEW

Additional Details : Weight : 0.09000kg

Product Code. 15360186

  • £620.00 / Each


Estimated Shipment date
from Supplier 26-06-2020
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Description

Description

The PolarScreen™ Glucocorticoid Receptor Competitor Assay Kit, Green, provides a sensitive and efficient method for high-throughput, fluorescence polarization-based screening of potential glucocorticoid receptor (GR) ligands. The kit uses insect cell-expressed, full-length, native (untagged) human GR (also available separately) and a novel, high-affinity, fluorescent glucocorticoid ligand (Fluormone™ GS1 Green) in a homogenous, mix-and-read assay format.

This kit produces a green color read-out; a 'red' kit is also available. Both kits suitably determine the IC50s of competitive compounds. Color choice depends on the spectral properties of the compounds to be screened and the available instrumentation. For additional information or assistance, contact us at drugdiscoverytech@lifetech.com.

The PolarScreen Glucocorticoid Receptor Competitor Assay is:

  • Complete & ready-to-use out of the box—all reagents included, just add your test compounds
  • High throughput compatible—fluorescence polarization assays in a 384-well plate
  • Convenient—components available in bulk sizes for larger screening needs

    How the Assay Works
    GR is added to a fluorescent glucocorticoid ligand to form a GR-Fluormone GS1 complex in the presence of competitor test compounds in microwell plates. The presence of effective competitors prevents the formation of a GS1/GR complex, resulting in a decrease of the polarization value. The shift in polarization value in the presence of test compounds is used to determine relative affinity of test compounds for GR. This assay is designed to be carried out at room temperature through use of a coactivator-related stabilizing peptide. Alternatively, the assay may be run at 4°C in the absence of the stabilizing peptide, with insignificant change in GR binding characteristics.

    Contact us for bulk quantities or for assistance with other nuclear receptor assay needs, from assay development to library screening and beyond.
  • Specifications

    Specifications

    Green
    Competitive Binding Assay, Nuclear Receptor Assay
    1 Tube Glucocorticoid Receptor Recombinant Protein, 1 tube of Fluormone GS1, 3 tubes of GR Screening Buffer, 3 tubes of GR Stabilizing Peptide, 1 tube of 1 M DTT
    PolarScreen™ Glucocorticoid Receptor Competitor Assay Kit
    400 x 20μL
    400 x 20μL
    1 Each
    PolarScreen™ Glucocorticoid Receptor Competitor Assay Kit, 400 x 20μL Assays, Green, Competitive Binding Assay, Nuclear Receptor Assay Validated Application, Dry Ice Storage Consideration, –80°C Immediate Storage, High Throughput Compatible
    384-well plate
    Yes
    PolarScreen™ Glucocorticoid Receptor Competitor Assay Kit
    For research use only. Not for use in diagnostic procedures
    1 Tube glucocorticoid receptor recombinant protein (store at –68 to –85°C), 1 tube of fluormone GS1 (store at –5 to –30°C), 3 tubes of GR screening buffer (store at –5 to –30°C), 3 tubes of GR stabilizing peptide (store at –68 to –85°C), 1 tube of 1 M DTT (store at –68 to –85°C)
    GR Screening buffer
    Videos
    Documents

    For Research Use Only. Not for use in diagnostic procedures.