Invitrogen™ PolarScreen™ ER Beta Competitor Assay Kit, Green

PolarScreen™ ER Beta Competitor Assay Kit, Green

Product Code	Brand	Quantity
15363826	Invitrogen™ A15890	800 Assays
15353826	Invitrogen™ A15889	2500 Assays

Description

The PolarScreen™ ER Beta Competitor Assay Kit, Green, provides a sensitive and efficient method for high-throughput, fluorescence polarization-based screening of potential estrogen receptor (ER) beta ligands. The kit uses insect cell-expressed, full-length, native (untagged) human ER beta and a novel, high-affinity, fluorescent estrogen ligand (Fluormone™ ES2) in a homogenous, mix-and-read assay format.

This kit produces a green color read-out; a 'red' kit is also available. Both kits suitably determine the IC50s of competitive compounds. Color choice depends on the spectral properties of the compounds to be screened and the available instrumentation. For additional information or assistance email drugdiscoverytech@lifetech.com.

The PolarScreen™ ER Beta Competitor Assay Kit is:

Complete & ready-to-use out of the box—all reagents included, just add your test compounds

High throughput compatible—20μL fluorescence polarization assays in a 384-well plate

Convenient—available in bulk sizes for larger screening needs

How the Assay Works
Full-length ER beta is added to a fluorescent estrogen ligand to form an ER-Fluormone™ ES2 complex. This complex is added to individual test compounds in multiwell plates. To determine relative affinity, the assay measures fluorescence polarization. Competing test compounds will displace the Fluormone™ ES2 ligand from ER beta, permitting it to tumble rapidly and resulting in low polarization values. A shift in the fluorescence polarization value in the presence of test compound is used to determine the relative affinity of test compounds for ER beta.

All components are manufactured under the strictest quality parameters. This product's performance, per the Certificate of Analysis, is guaranteed for 6 months after purchase. Contact us for bulk quantities or for assistance with other nuclear receptor assay needs, from assay development to library screening and beyond.

Specifications

• Color: Green
• Packaging: 384-well Plate

For Research Use Only. Not for use in diagnostic procedures.